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PMID: 1498607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Homodimeric and heterodimeric leucine zipper proteins and nuclear factors from parsley recognize diverse promoter elements with ACGT cores.

The Plant cell ·Vol. 4 ·No. 5 ·1992-05-00 ·Pages 525-37

Armstrong GA, Weisshaar B, Hahlbrock K

Abstract

Four short nucleotide sequences (boxes I to IV) contribute to the light responsiveness of the parsley chalcone synthase promoter. The sequence-related boxes II and III resemble several plant, viral, and bacterial promoter elements that share ACGT core sequences and are associated with diversely regulated genes. We have analyzed the binding characteristics and protein-protein interactions of factors from nuclear extracts and of three putative leucine zipper (bZIP) transcription factors potentially involved in the regulation of this promoter. These common plant regulatory factors (CPRFs) bind specifically to boxes II and III as well as other ACGT-containing promoter elements (hex1, Em1a, and as-1), though with markedly different affinities. Intact bZIP domains are crucial for CPRF binding to DNA. Distinct ensembles of nuclear factors bind to boxes II and III, despite their sequence similarity. The parsley CPRFs bind to DNA as dimers, selectively form heterodimeric DNA binding complexes, and interact with nuclear proteins.

MeSH Terms
Acyltransferases/genetics Amino Acid Sequence Base Sequence DNA DNA-Binding Proteins/metabolism Gene Expression Regulation, Enzymologic/radiation effects Leucine Zippers Light Molecular Sequence Data Nuclear Proteins/metabolism Plant Proteins/metabolism Plants/genetics Promoter Regions, Genetic
Chemicals
CPRF protein, Petroselinum crispum DNA-Binding Proteins Nuclear Proteins Plant Proteins DNA Acyltransferases flavanone synthetase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Armstrong G A
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.
Weisshaar B
Hahlbrock K
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35 references, click to expand
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.4.5.525
Published
1992-05-00
Pages
525-37
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160150
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.4.5.525 PMC Full Text View journal details
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