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PMID: 1500503 Published · ppublish English Case Reports Journal Article Research Support, Non-U.S. Gov't

Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates.

Journal of clinical microbiology ·Vol. 30 ·No. 8 ·1992-08-00 ·Pages 1982-4

Salama SM, Tabor H, Richter M, Taylor DE

Abstract

Campylobacter hyointestinalis was isolated from five members of the same family who had previously consumed raw milk. Pulsed-field gel electrophoresis of genomic DNAs from the five strains, after digestion with restriction endonuclease SalI, revealed that three strains had identical genome patterns and therefore appeared to be related, whereas the other two had completely different genome patterns and appeared to be unrelated. We report here for the first time the isolation of C. hyointestinalis from family members who had consumed raw milk. Our study also demonstrates the usefulness of pulsed-field gel electrophoresis for epidemiologic studies of this unusual campylobacter.

MeSH Terms
Bacteriological Techniques Campylobacter/classification,genetics,isolation & purification Campylobacter Infections/epidemiology,microbiology DNA, Bacterial/genetics Electrophoresis, Gel, Pulsed-Field/methods Evaluation Studies as Topic Female Humans Infant Species Specificity
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salama S M
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Tabor H
Richter M
Taylor D E
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18 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.8.1982-1984.1992
Published
1992-08-00
Pages
1982-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265427
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.8.1982-1984.1992 PMC Full Text View journal details
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