Home LiteratureArticle Details
PMID: 15020433 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

High-density linkage mapping revealed suppression of recombination at the sex determination locus in papaya.

Genetics ·Vol. 166 ·No. 1 ·2004-01-00 ·Pages 419-36

Ma H, Moore PH, Liu Z, Kim MS, Yu Q, Fitch MM, Sekioka T, Paterson AH, Ming R

Abstract

A high-density genetic map of papaya (Carica papaya L.) was constructed using 54 F(2) plants derived from cultivars Kapoho and SunUp with 1501 markers, including 1498 amplified fragment length polymorphism (AFLP) markers, the papaya ringspot virus coat protein marker, morphological sex type, and fruit flesh color. These markers were mapped into 12 linkage groups at a LOD score of 5.0 and recombination frequency of 0.25. The 12 major linkage groups covered a total length of 3294.2 cM, with an average distance of 2.2 cM between adjacent markers. This map revealed severe suppression of recombination around the sex determination locus with a total of 225 markers cosegregating with sex types. The cytosine bases were highly methylated in this region on the basis of the distribution of methylation-sensitive and -insensitive markers. This high-density genetic map is essential for cloning of specific genes of interest such as the sex determination gene and for the integration of genetic and physical maps of papaya.

MeSH Terms
Base Composition Carica/genetics Chromosome Mapping Chromosomes, Plant/genetics DNA Methylation DNA, Plant/chemistry,genetics Genes, Plant Genome, Plant Polymorphism, Genetic Recombination, Genetic Sex Determination Processes Suppression, Genetic
Chemicals
DNA, Plant
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ma Hao
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii 96822, USA.
Moore Paul H
Liu Zhiyong
Kim Minna S
Yu Qingyi
Fitch Maureen M M
Sekioka Terry
Paterson Andrew H
Ming Ray
References (30)
30 references, click to expand
  1. A high-density cytogenetic map of the Aegilops tauschii genome incorporating retrotransposons and defense-related genes: insights into cereal chromosome structure and function.
    Plant Mol Biol. 2002 Mar-Apr;48(5-6):767-90 PMID: 11999849
  2. A physical amplified fragment-length polymorphism map of Arabidopsis.
    Plant Physiol. 2001 Dec;127(4):1579-89 PMID: 11743102
  3. High density molecular linkage maps of the tomato and potato genomes.
    Genetics. 1992 Dec;132(4):1141-60 PMID: 1360934
  4. Genetic diversity of Carica papaya as revealed by AFLP markers.
    Genome. 2002 Jun;45(3):503-12 PMID: 12033619
  5. AFLP: a new technique for DNA fingerprinting.
    Nucleic Acids Res. 1995 Nov 11;23(21):4407-14 PMID: 7501463
  6. Tight clustering and hemizygosity of apomixis-linked molecular markers in Pennisetum squamulatum implies genetic control of apospory by a divergent locus that may have no allelic form in sexual genotypes.
    Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5127-32 PMID: 9560240
  7. Genetic definition and sequence analysis of Arabidopsis centromeres.
    Science. 1999 Dec 24;286(5449):2468-74 PMID: 10617454
  8. Diagnostic criteria for primary osteoporosis: year 2000 revision.
    J Bone Miner Metab. 2001;19(6):331-7 PMID: 11685647
  9. Control of papaya ringspot virus in papaya: a case study.
    Annu Rev Phytopathol. 1998;36:415-37 PMID: 15012507
  10. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.
    Genomics. 1987 Oct;1(2):174-81 PMID: 3692487
  11. Arabidopsis thaliana centromere regions: genetic map positions and repetitive DNA structure.
    Genome Res. 1997 Nov;7(11):1045-53 PMID: 9371740
  12. AFLP analysis of genetic diversity within and among Coffea arabica cultivars.
    Theor Appl Genet. 2002 Aug;105(2-3):209-215 PMID: 12582521
  13. A high-throughput AFLP-based method for constructing integrated genetic and physical maps: progress toward a sorghum genome map.
    Genome Res. 2000 Jun;10(6):789-807 PMID: 10854411
  14. Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes.
    Trends Genet. 1995 Feb;11(2):63-8 PMID: 7716809
  15. CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower.
    Plant Mol Biol. 1991 Jul;17(1):111-23 PMID: 1831056
  16. Suppression of intrachromosomal gene conversion in mammalian cells by small degrees of sequence divergence.
    Genetics. 1999 Apr;151(4):1559-68 PMID: 10101177
  17. A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers.
    Plant Mol Biol. 2002 Mar-Apr;48(5-6):483-99 PMID: 11999830
  18. A high-density rice genetic linkage map with 2275 markers using a single F2 population.
    Genetics. 1998 Jan;148(1):479-94 PMID: 9475757
  19. A male and hermaphrodite specific RAPD marker for papaya ( Carica papayaL.).
    Theor Appl Genet. 2002 Feb;104(2-3):281-285 PMID: 12582698
  20. Level of polymorphism and genetic mapping of AFLP markers in rice.
    Genome. 1996 Oct;39(5):969-77 PMID: 8890522
  21. Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
    Science. 1993 Nov 26;262(5138):1432-6 PMID: 7902614
  22. A comprehensive rice transcript map containing 6591 expressed sequence tag sites.
    Plant Cell. 2002 Mar;14(3):525-35 PMID: 11910001
  23. RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato.
    Genetics. 1988 Dec;120(4):1095-103 PMID: 17246486
  24. Molecular markers for sex determination in papaya ( Carica papaya L.).
    Theor Appl Genet. 2002 Dec;106(1):107-11 PMID: 12582877
  25. A maize map standard with sequenced core markers, grass genome reference points and 932 expressed sequence tagged sites (ESTs) in a 1736-locus map.
    Genetics. 1999 Jul;152(3):1137-72 PMID: 10388831
  26. Correlation of the physical and genetic maps of the centromeric region of the right arm of linkage group III of Neurospora crassa.
    Genetics. 1994 Apr;136(4):1297-306 PMID: 7912215
  27. Comparative genomics of plant chromosomes.
    Plant Cell. 2000 Sep;12(9):1523-40 PMID: 11006329
  28. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  29. Toward integration of comparative genetic, physical, diversity, and cytomolecular maps for grasses and grains, using the sorghum genome as a foundation.
    Plant Physiol. 2001 Mar;125(3):1325-41 PMID: 11244113
  30. Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population.
    Plant J. 1998 Apr;14(2):259-71 PMID: 9628021
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.166.1.419
Published
2004-01-00
Pages
419-36
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1470706
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.166.1.419 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2