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PMID: 1502152 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification of the heteromeric protein binding to the URS1 transcriptional repression site in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 16 ·1992-08-15 ·Pages 7412-6

Luche RM, Smart WC, Cooper TG

Abstract

The protein that binds to the URS1 site situated upstream of many genes in Saccharomyces cerevisiae is a central element responsible for global negative control of transcription in this organism. Among the genes whose expression is regulated by this protein are those that participate in nitrogen metabolism, carbon metabolism, electron transport, inositol metabolism, heat shock response, meiosis, and sporulation. This factor, binding URS1 factor (BUF), has been purified and shown to be a heteromeric protein composed of 37.5- and 73.5-kDa monomers. The heteromeric form of BUF is stably maintained both in solution and bound to its DNA target site.

Related Genes
CAR1
MeSH Terms
Arginase/genetics Base Sequence Carrier Proteins/isolation & purification,metabolism DNA, Fungal/genetics,isolation & purification Fungal Proteins/isolation & purification,metabolism Genes, Fungal Genes, Regulator Macromolecular Substances Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
Carrier Proteins DNA, Fungal Fungal Proteins Macromolecular Substances Oligodeoxyribonucleotides Arginase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luche R M
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Smart W C
Cooper T G
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.16.7412
Published
1992-08-15
Pages
7412-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49720
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-35642 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.89.16.7412 PMC Full Text View journal details
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