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PMID: 15060024 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Metabolic activity of Staphylococcus epidermidis is high during initial and low during late experimental foreign-body infection.

Journal of bacteriology ·Vol. 186 ·No. 8 ·2004-04-00 ·Pages 2236-9

Vandecasteele SJ, Peetermans WE, Carbonez A, Van Eldere J

Abstract

Foreign-body infection (FBI) is notoriously resistant to eradication by antibiotic treatment. It is hypothesized that reduced bacterial metabolic activity contributes to this resistance. We examined the metabolic activity of Staphylococcus epidermidis in 204 samples recovered during in vitro foreign-body colonization and in 424 samples recovered during in vivo FBI in a rat model. Metabolic activity was measured by determining the amount of 16S rRNA per genome by quantitative PCR. The initial foreign-body-associated growth proved to be a metabolically active process, both in vitro and in vivo. The initial 16S rRNA content was similar to that observed during in vitro exponential-growth phase. However, during late in vivo FBI, a 114-fold (P << 0.0001) decrease in the 16S rRNA content was observed, indicating that there was markedly decreased metabolic activity. This decreased metabolic activity during late FBI can explain at least in part why such infections are so difficult to eradicate with conventional antibiotic treatment.

MeSH Terms
Animals Catheters, Indwelling/microbiology Disease Models, Animal Foreign-Body Reaction/microbiology Germ-Free Life Polymerase Chain Reaction Polyurethanes RNA, Bacterial/analysis RNA, Ribosomal, 16S/analysis Rats Rats, Inbred F344 Staphylococcal Infections/microbiology Staphylococcus epidermidis/genetics,metabolism Time Factors
Chemicals
Polyurethanes RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vandecasteele Stefaan Johan
Infectious Diseases Research Group, Department of Microbiology and Immunology, Rega Institute for Medical Research and Internal Medicine and Infectious Diseases, University Hospital Leuven, Leuven, Belgium. [email protected]
Peetermans Willy Eduard
Carbonez An
Van Eldere Johan
References (28)
28 references, click to expand
  1. Stringent control of bacterial transcription.
    Cell. 1985 May;41(1):6-8 PMID: 2581696
  2. Effect of biofilm culture upon the susceptibility of Staphylococcus epidermidis to tobramycin.
    J Antimicrob Chemother. 1992 Dec;30(6):803-10 PMID: 1289354
  3. p values for pathogens: statistical inference from infectious-disease data.
    Lancet Infect Dis. 2002 Apr;2(4):219-30 PMID: 11937422
  4. Starvation recovery of Staphylococcus aureus 8325-4.
    Microbiology. 1998 Jul;144 ( Pt 7):1755-63 PMID: 9695909
  5. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
  6. The timing of prophylactic administration of antibiotics and the risk of surgical-wound infection.
    N Engl J Med. 1992 Jan 30;326(5):281-6 PMID: 1728731
  7. Expression of biofilm-associated genes in Staphylococcus epidermidis during in vitro and in vivo foreign body infections.
    J Infect Dis. 2003 Sep 1;188(5):730-7 PMID: 12934189
  8. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
    Science. 1989 Mar 10;243(4896):1360-3 PMID: 2466341
  9. Resistance of Staphylococcus aureus recovered from infected foreign body in vivo to killing by antimicrobials.
    J Infect Dis. 1991 Jun;163(6):1369-73 PMID: 1903801
  10. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces.
    Infect Immun. 1982 Jul;37(1):318-26 PMID: 6179880
  11. The development of a small-scale biofilm model suitable for studying the effects of antibiotics on biofilms of gram-negative bacteria.
    J Antimicrob Chemother. 1997 Sep;40(3):329-34 PMID: 9338483
  12. Spatial variations in growth rate within Klebsiella pneumoniae colonies and biofilm.
    Biotechnol Prog. 1996 May-Jun;12(3):316-21 PMID: 8652119
  13. Quantification of bacterial transcripts during infection using competitive reverse transcription-PCR (RT-PCR) and LightCycler RT-PCR.
    Clin Diagn Lab Immunol. 2001 Mar;8(2):279-82 PMID: 11238208
  14. Antibiotic resistance of bacteria in biofilms.
    Lancet. 2001 Jul 14;358(9276):135-8 PMID: 11463434
  15. The regulation of transcription initiation in bacteria.
    Annu Rev Genet. 1985;19:355-87 PMID: 3936407
  16. Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters.
    J Infect Dis. 1982 Oct;146(4):479-82 PMID: 7119478
  17. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions.
    J Bacteriol. 2001 Dec;183(24):7094-101 PMID: 11717267
  18. Foreign body infection: a new rat model for prophylaxis and treatment.
    J Antimicrob Chemother. 1999 Nov;44(5):669-74 PMID: 10552984
  19. Central venous catheters coated with minocycline and rifampin for the prevention of catheter-related colonization and bloodstream infections. A randomized, double-blind trial. The Texas Medical Center Catheter Study Group.
    Ann Intern Med. 1997 Aug 15;127(4):267-74 PMID: 9265425
  20. Impact of the regulatory loci agr, sarA and sae of Staphylococcus aureus on the induction of alpha-toxin during device-related infection resolved by direct quantitative transcript analysis.
    Mol Microbiol. 2001 Jun;40(6):1439-47 PMID: 11442841
  21. Estimating the growth rate of slowly growing marine bacteria from RNA content.
    Appl Environ Microbiol. 1993 Aug;59(8):2594-601 PMID: 16349018
  22. A method to isolate RNA from gram-positive bacteria and mycobacteria.
    Anal Biochem. 1994 Nov 1;222(2):511-4 PMID: 7532381
  23. Use of gDNA as internal standard for gene expression in staphylococci in vitro and in vivo.
    Biochem Biophys Res Commun. 2002 Mar 1;291(3):528-34 PMID: 11855820
  24. Induction of staphylococcal infections in mice with small inocula introduced on sutures.
    Br J Exp Pathol. 1961 Jun;42:266-77 PMID: 13789320
  25. Susceptibility of Staphylococcus aureus growing on fibronectin-coated surfaces to bactericidal antibiotics.
    Antimicrob Agents Chemother. 1993 Apr;37(4):625-32 PMID: 8494358
  26. Bactericidal effects of antibiotics on slowly growing and nongrowing bacteria.
    Antimicrob Agents Chemother. 1991 Sep;35(9):1824-8 PMID: 1952852
  27. A comparison of two antimicrobial-impregnated central venous catheters. Catheter Study Group.
    N Engl J Med. 1999 Jan 7;340(1):1-8 PMID: 9878638
  28. Transcription of clumping factor A in attached and unattached Staphylococcus aureus in vitro and during device-related infection.
    Infect Immun. 2002 Jun;70(6):2758-62 PMID: 12010960
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.8.2236-2239.2004
Email
[email protected]
Published
2004-04-00
Pages
2236-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC412167
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.186.8.2236-2239.2004 PMC Full Text View journal details
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