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PMID: 15060170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.

Molecular and cellular biology ·Vol. 24 ·No. 8 ·2004-04-00 ·Pages 3514-25

Vadivelu SK, Kurzbauer R, Dieplinger B, Zweyer M, Schafer R, Wernig A, Vietor I, Huber LA

Abstract

The tetradecanoyl phorbol acetate-induced sequence 7 gene (tis7) is regulated during cell fate processes and functions as a transcriptional coregulator. Here, we describe the generation and analysis of mice lacking the tis7 gene. Surprisingly, TIS7 knockout mice show no gross histological abnormalities and are fertile. Disruption of the tis7 gene by homologous recombination delayed muscle regeneration and altered the isometric contractile properties of skeletal muscles after muscle crush damage in TIS7(-/-) mice. Cultured primary myogenic satellite cells (MSCs) from TIS7(-/-) mice displayed marked reductions in differentiation potential and fusion index in a strictly cell-autonomous fashion. Loss of TIS7 caused the down-regulation of muscle-specific genes, such as those for MyoD, myogenin, and laminin-alpha2. Fusion potential in TIS7(-/-) MSCs could be rescued by TIS7 expression or laminin supplementation. Therefore, TIS7 is not essential for mouse development but plays a novel regulatory role during adult muscle regeneration.

MeSH Terms
Animals Cell Differentiation/physiology Cells, Cultured Coculture Techniques Fluorescent Dyes/metabolism Gene Targeting Immediate-Early Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Mice Mice, Knockout Muscle Contraction/physiology Muscle, Skeletal/cytology,physiology Myoblasts/cytology,physiology Phenotype Regeneration/physiology
Chemicals
Fluorescent Dyes Ifrd1 protein, mouse Immediate-Early Proteins Membrane Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Vadivelu Santhosh K
Institute for Anatomy, Histology, and Embryology, Department of Histology and Molecular Cell Biology, Medical University Innsbruck, A-6020 Innsbruck, Austria.
Kurzbauer Robert
Dieplinger Benjamin
Zweyer Margit
Schafer Ralf
Wernig Anton
Vietor Ilja
Huber Lukas A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.8.3514-3525.2004
Published
2004-04-00
Pages
3514-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC381666
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/MCB.24.8.3514-3525.2004 PMC Full Text View journal details
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