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PMID: 15079051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 16 ·2004-04-20 ·Pages 6309-14

Mukhopadhyay A, Vij S, Tyagi AK

Abstract

Stress perception and signal transduction leading to tolerance involve a complex interplay of different gene products. We describe here the isolation and characterization of an intronless gene (OSISAP1) from rice encoding a zinc-finger protein that is induced after different types of stresses, namely cold, desiccation, salt, submergence, and heavy metals as well as injury. The gene is also induced by stress hormone abscisic acid. Overexpression of the gene in transgenic tobacco conferred tolerance to cold, dehydration, and salt stress at the seed-germination/seedling stage as reflected by the percentage of germination/green seedlings, the fresh weight of seedlings, and their developmental pattern. Thus, OSISAP1 seems to be an important determinant of stress response in plants.

MeSH Terms
Adaptation, Physiological/genetics Amino Acid Sequence DNA, Complementary Molecular Sequence Data Oryza/genetics,physiology Plant Proteins/chemistry,genetics,physiology Plants, Genetically Modified/genetics,physiology Sequence Homology, Amino Acid Sodium Chloride Tobacco/genetics,physiology Water Zinc Fingers
Chemicals
DNA, Complementary Plant Proteins Water Sodium Chloride
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mukhopadhyay Arnab
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India.
Vij Shubha
Tyagi Akhilesh K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0401572101
Published
2004-04-20
Epub
2004-00-12
Pages
6309-14
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC395965
Subset
IM
Databases
GENBANK
AF137590, AF140722
External Links
PubMed source DOI: 10.1073/pnas.0401572101 PMC Full Text View journal details
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