Abstract
In the current studies we generated transgenic mice that overexpress human Insig-1 in the liver under a constitutive promoter. In cultured cells Insig-1 and Insig-2 have been shown to block lipid synthesis in a cholesterol-dependent fashion by inhibiting proteolytic processing of sterol regulatory element-binding proteins (SREBPs), membrane-bound transcription factors that activate lipid synthesis. Insig's exert this action in the ER by binding SREBP cleavage-activating protein (SCAP) and preventing it from escorting SREBPs to the Golgi apparatus where the SREBPs are processed to their active forms. In the livers of Insig-1 transgenic mice, the content of all nuclear SREBPs (nSREBPs) was reduced and declined further upon feeding of dietary cholesterol. The nuclear content of the insulin-induced SREBP isoform, SREBP-1c, failed to increase to a normal extent upon refeeding on a high-carbohydrate diet. The nSREBP deficiency produced a marked reduction in the levels of mRNAs encoding enzymes required for synthesis of cholesterol, fatty acids, and triglycerides. Plasma cholesterol levels were strongly reduced, and plasma triglycerides did not exhibit their normal rise after refeeding. These results provide in vivo support for the hypothesis that nSREBPs are essential for high levels of lipid synthesis in the liver and indicate that Insig's modulate nSREBP levels by binding and retaining SCAP in the ER.
MeSH Terms
Animals
CCAAT-Enhancer-Binding Proteins/genetics,metabolism
DNA-Binding Proteins/genetics,metabolism
Humans
Insulin/pharmacology
Intracellular Signaling Peptides and Proteins
Lipids/biosynthesis
Liver/metabolism
Membrane Proteins/physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
RNA, Messenger/analysis
Sterol Regulatory Element Binding Protein 1
Transcription Factors
Triglycerides/blood
Chemicals
CCAAT-Enhancer-Binding Proteins
DNA-Binding Proteins
INSIG1 protein, human
Insulin
Intracellular Signaling Peptides and Proteins
Lipids
Membrane Proteins
RNA, Messenger
SREBF1 protein, human
SREBP cleavage-activating protein
Srebf1 protein, mouse
Sterol Regulatory Element Binding Protein 1
Transcription Factors
Triglycerides
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Engelking Luke J
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, 75390, USA.
Kuriyama Hiroshi
Hammer Robert E
Horton Jay D
Brown Michael S
Goldstein Joseph L
Liang Guosheng
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