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PMID: 15121899 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

APOBEC3G is a single-stranded DNA cytidine deaminase and functions independently of HIV reverse transcriptase.

Nucleic acids research ·Vol. 32 ·No. 8 ·2004-00-00 ·Pages 2421-9

Suspène R, Sommer P, Henry M, Ferris S, Guétard D, Pochet S, Chester A, Navaratnam N, Wain-Hobson S, Vartanian JP

Abstract

In the absence of the viral vif gene, human immunodeficiency virus (HIV) may be restricted by the APOBEC3G gene on chromosome 22. The role of the HIV Vif protein is to exclude host cell APOBEC3G from the budding virion. As APOBEC3G shows sequence homology to cytidine deaminases, it is presumed that in the absence of Vif, cytidine residues in the cDNA are deaminated yielding uracil. It is not known if additional proteins mediate APOBEC3G function or if deamination occurs in concert with reverse transcription. This report describes an in vitro assay showing that Baculovirus derived APOBEC3G alone extensively deaminates cDNA independently of reverse transcriptase. It reproduces the dinucleotide context typical of G --> A hypermutants derived from a Delta(vif) virus. By using an RNaseH- form of reverse transcriptase, it was shown that the cDNA has to be free of its RNA template to allow deamination. APOBEC3G deamination of dC or dCTP was not detected. In short, APOBEC3G is a single-stranded DNA cytidine deaminase capable of restricting retroviral replication.

MeSH Terms
APOBEC-3G Deaminase Base Sequence Cytidine Deaminase/metabolism DNA, Single-Stranded/metabolism Dinucleoside Phosphates/metabolism HIV Reverse Transcriptase/metabolism Nucleoside Deaminases Proteins/metabolism,physiology Repressor Proteins Substrate Specificity Transcription, Genetic
Chemicals
DNA, Single-Stranded Dinucleoside Phosphates Proteins Repressor Proteins cytidylyl-(3'-5')-cytidine HIV Reverse Transcriptase Nucleoside Deaminases APOBEC-3G Deaminase APOBEC3G protein, human Cytidine Deaminase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Suspène Rodolphe
Unité de Rétrovirologie Moléculaire, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France.
Sommer Peter
Henry Michel
Ferris Stéphane
Guétard Denise
Pochet Sylvie
Chester Ann
Navaratnam Naveenan
Wain-Hobson Simon
Vartanian Jean-Pierre
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh554
Published
2004-00-00
Epub
2004-00-30
Pages
2421-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC419444
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkh554 PMC Full Text View journal details
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