Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation.

Nature biotechnology ·Vol. 22 ·No. 6 ·2004-06-00 ·Pages 707-16

Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW

Abstract

Human embryonic stem (hES) cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the molecular level, we obtained 148,453 expressed sequence tags (ESTs) from undifferentiated hES cells and three differentiated derivative subpopulations. Over 32,000 different transcripts expressed in hES cells were identified, of which more than 16,000 do not match closely any gene in the UniGene public database. Queries to this EST database revealed 532 significantly upregulated and 140 significantly downregulated genes in undifferentiated hES cells. These data highlight changes in the transcriptional network that occur when hES cells differentiate. Among the differentially regulated genes are several components of signaling pathways and transcriptional regulators that likely play key roles in hES cell growth and differentiation. The genomic data presented here may facilitate the derivation of clinically useful cell types from hES cells.

MeSH Terms

Antigens, CD/genetics,physiology Cell Differentiation/drug effects,genetics,physiology Cell Division/drug effects,genetics,physiology Cell Line Cytokine Receptor gp130 Dimethyl Sulfoxide/pharmacology Down-Regulation/drug effects,genetics Embryo, Mammalian/cytology Expressed Sequence Tags Fibroblast Growth Factors/genetics,physiology Gene Expression/drug effects Gene Expression Profiling Gene Library Growth Substances/pharmacology Humans Intercellular Signaling Peptides and Proteins/genetics,physiology Interleukin-6 Leukemia Inhibitory Factor Membrane Glycoproteins/genetics,physiology Nodal Protein Proteins/genetics,physiology Proto-Oncogene Proteins/genetics,physiology RNA/genetics,isolation & purification Receptors, Fibroblast Growth Factor/genetics,physiology Receptors, G-Protein-Coupled/genetics,physiology Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Signal Transduction/genetics,physiology Stem Cells/cytology,metabolism Transcription Factors/genetics,physiology Transcription, Genetic/genetics Transforming Growth Factor beta/genetics,physiology Tretinoin/pharmacology Up-Regulation/drug effects,genetics Wnt Proteins

Chemicals

Antigens, CD Growth Substances IL6ST protein, human Intercellular Signaling Peptides and Proteins Interleukin-6 LIF protein, human Leukemia Inhibitory Factor Membrane Glycoproteins NODAL protein, human Nodal Protein Proteins Proto-Oncogene Proteins Receptors, Fibroblast Growth Factor Receptors, G-Protein-Coupled Transcription Factors Transforming Growth Factor beta Wnt Proteins Cytokine Receptor gp130 Tretinoin Fibroblast Growth Factors RNA Dimethyl Sulfoxide

Authors & Affiliations

14 authors, click to expand affiliations / ORCID

Brandenberger Ralph

Geron Corporation, Menlo Park, California 94025, USA. [email protected]

Wei Henry

Zhang Sally

Lei Shirley

Murage Jaji

Fisk Gregory J

Li Yan

Xu Chunhui

Fang Rixun

Guegler Karl

Rao Mahendra S

Mandalam Ramumkar

Lebkowski Jane

Stanton Lawrence W

Article Info

Journal

Nature biotechnology

Abbr.

Nat Biotechnol

ISSN

1087-0156

DOI

10.1038/nbt971

[email protected]

Published

2004-06-00

Epub

2004-00-16

Pages

707-16

Language

English

Region

United States

NLM ID

9604648

Subset

External Links

PubMed source DOI: 10.1038/nbt971 View journal details

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Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation.

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