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PMID: 1516818 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of Caenorhabditis elegans lectin-binding mutants.

Genetics ·Vol. 131 ·No. 4 ·1992-08-00 ·Pages 867-81

Link CD, Silverman MA, Breen M, Watt KE, Dames SA

Abstract

We have identified 45 mutants of Caenorhabditis elegans that show ectopic surface binding of the lectins wheat germ agglutinin (WGA) and soybean agglutinin (SBA). These mutations are all recessive and define six genes: srf-2, srf-3, srf-4, srf-5, srf-8 and srf-9. Mutations in these genes fall into two phenotypic classes: srf-2, -3, -5 mutants are grossly wild-type, except for their lectin-binding phenotype; srf-4, -8, -9 mutants have a suite of defects, including uncoordinated movement, abnormal egg laying, and defective copulatory bursae morphogenesis. Characterization of these pleiotropic mutants at the cellular level reveals defects in the migration of the gonadal distal tip cell and in axon morphology. Unexpectedly, the pleiotropic mutations also interact with mutations in the lin-12 gene, which encodes a putative cell surface receptor involved in the control of cell fate. We propose that the underlying defect in the pleiotropic mutations may be in the general processing or secretion of extracellular proteins.

Related Genes
lin-12 srf-2 srf-3 srf-4 srf-5 srf-8 srf-9
MeSH Terms
Animals Caenorhabditis/genetics,metabolism Chromosome Mapping Immunohistochemistry Lectins/metabolism Mutation Phenotype Plant Lectins Soybean Proteins Wheat Germ Agglutinins/metabolism
Chemicals
Lectins Plant Lectins Soybean Proteins Wheat Germ Agglutinins soybean lectin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Link C D
Department of Biological Sciences, University of Denver, Colorado 80208.
Silverman M A
Breen M
Watt K E
Dames S A
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45 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/131.4.867
Published
1992-08-00
Pages
867-81
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205098
Subset
IM
Grants
NICHD NIH HHS · R29 HD26087 · United States
External Links
PubMed source DOI: 10.1093/genetics/131.4.867 PMC Full Text View journal details
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