Home LiteratureArticle Details
PMID: 15175386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 24 ·No. 22 ·2004-06-02 ·Pages 5172-6

Ashby MC, De La Rue SA, Ralph GS, Uney J, Collingridge GL, Henley JM

Abstract

AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.

MeSH Terms
Animals Cell Membrane/metabolism Cells, Cultured Endocytosis/physiology Green Fluorescent Proteins Hippocampus/cytology Hydrogen-Ion Concentration Luminescent Proteins/genetics Microscopy, Fluorescence Neurons/metabolism Protein Transport/physiology Rats Receptors, AMPA/genetics,metabolism Receptors, N-Methyl-D-Aspartate/metabolism Recombinant Fusion Proteins/genetics,metabolism Synapses/metabolism
Chemicals
Luminescent Proteins Receptors, AMPA Receptors, N-Methyl-D-Aspartate Recombinant Fusion Proteins Green Fluorescent Proteins glutamate receptor ionotropic, AMPA 2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ashby Michael C
Medical Research Council, Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom.
De La Rue Sarah A
Ralph G Scott
Uney James
Collingridge Graham L
Henley Jeremy M
References (21)
21 references, click to expand
  1. LTP mechanisms: from silence to four-lane traffic.
    Curr Opin Neurobiol. 2000 Jun;10(3):352-7 PMID: 10851179
  2. The use of pHluorins for optical measurements of presynaptic activity.
    Biophys J. 2000 Oct;79(4):2199-208 PMID: 11023924
  3. Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD.
    Nat Neurosci. 2000 Dec;3(12):1291-300 PMID: 11100150
  4. Reinsertion or degradation of AMPA receptors determined by activity-dependent endocytic sorting.
    Neuron. 2000 Nov;28(2):511-25 PMID: 11144360
  5. Role of AMPA receptor endocytosis in synaptic plasticity.
    Nat Rev Neurosci. 2001 May;2(5):315-24 PMID: 11331915
  6. Subunit-specific rules governing AMPA receptor trafficking to synapses in hippocampal pyramidal neurons.
    Cell. 2001 May 4;105(3):331-43 PMID: 11348590
  7. AMPA receptor trafficking and the control of synaptic transmission.
    Cell. 2001 Jun 29;105(7):825-8 PMID: 11439178
  8. Subunit-specific temporal and spatial patterns of AMPA receptor exocytosis in hippocampal neurons.
    Nat Neurosci. 2001 Sep;4(9):917-26 PMID: 11528423
  9. pH Homeostasis of cellular organelles.
    News Physiol Sci. 2002 Feb;17:1-5 PMID: 11821527
  10. Regulation of AMPA receptor lateral movements.
    Nature. 2002 Jun 6;417(6889):649-53 PMID: 12050666
  11. AMPA receptor trafficking and synaptic plasticity.
    Annu Rev Neurosci. 2002;25:103-26 PMID: 12052905
  12. Differential regulation of synaptic and extra-synaptic NMDA receptors.
    Nat Neurosci. 2002 Sep;5(9):833-4 PMID: 12195433
  13. Dynamics and regulation of clathrin coats at specialized endocytic zones of dendrites and spines.
    Neuron. 2002 Oct 24;36(3):435-49 PMID: 12408846
  14. Clathrin adaptor AP2 and NSF interact with overlapping sites of GluR2 and play distinct roles in AMPA receptor trafficking and hippocampal LTD.
    Neuron. 2002 Nov 14;36(4):661-74 PMID: 12441055
  15. The role of receptor diffusion in the organization of the postsynaptic membrane.
    Nat Rev Neurosci. 2003 Apr;4(4):251-65 PMID: 12671642
  16. Direct imaging of lateral movements of AMPA receptors inside synapses.
    EMBO J. 2003 Sep 15;22(18):4656-65 PMID: 12970178
  17. Tetracycline-regulated transgene expression in hippocampal neurones following transfection with adenoviral vectors.
    J Neurochem. 1997 Dec;69(6):2620-3 PMID: 9375698
  18. Activity differentially regulates the surface expression of synaptic AMPA and NMDA glutamate receptors.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7097-102 PMID: 9618545
  19. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins.
    Nature. 1998 Jul 9;394(6689):192-5 PMID: 9671304
  20. NMDA induces long-term synaptic depression and dephosphorylation of the GluR1 subunit of AMPA receptors in hippocampus.
    Neuron. 1998 Nov;21(5):1151-62 PMID: 9856470
  21. Nomadic AMPA receptors and LTP.
    Neuron. 1999 Jul;23(3):431-4 PMID: 10433256
Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.1042-04.2004
Published
2004-06-02
Pages
5172-6
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC3309030
Subset
IM
Grants
Wellcome Trust · United Kingdom
Wellcome Trust · 069280 · United Kingdom
Medical Research Council · G9629038 · United Kingdom
Medical Research Council · G9629038(61600) · United Kingdom
External Links
PubMed source DOI: 10.1523/JNEUROSCI.1042-04.2004 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2