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PMID: 15181204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Gene expression signatures from three genetically separable resistance gene signaling pathways for downy mildew resistance.

Plant physiology ·Vol. 135 ·No. 2 ·2004-06-00 ·Pages 1129-44

Eulgem T, Weigman VJ, Chang HS, McDowell JM, Holub EB, Glazebrook J, Zhu T, Dangl JL

Abstract

Resistance gene-dependent disease resistance to pathogenic microorganisms is mediated by genetically separable regulatory pathways. Using the GeneChip Arabidopsis genome array, we compared the expression profiles of approximately 8,000 Arabidopsis genes following activation of three RPP genes directed against the pathogenic oomycete Peronospora parasitica. Judicious choice of P. parasitica isolates and loss of resistance plant mutants allowed us to compare the responses controlled by three genetically distinct resistance gene-mediated signaling pathways. We found that all three pathways can converge, leading to up-regulation of common sets of target genes. At least two temporal patterns of gene activation are triggered by two of the pathways examined. Many genes defined by their early and transient increases in expression encode proteins that execute defense biochemistry, while genes exhibiting a sustained or delayed expression increase predominantly encode putative signaling proteins. Previously defined and novel sequence motifs were found to be enriched in the promoters of genes coregulated by the local defense-signaling network. These putative promoter elements may operate downstream from signal convergence points.

MeSH Terms
Arabidopsis/genetics,growth & development,microbiology Arabidopsis Proteins/genetics,metabolism Carboxylic Ester Hydrolases/genetics,metabolism Conserved Sequence/genetics Gene Expression Profiling/methods Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Immunity, Innate/genetics Peronospora/growth & development Phylogeny Plant Diseases/genetics,microbiology Signal Transduction/genetics Transcriptional Activation
Chemicals
Arabidopsis Proteins RPP5 protein, Arabidopsis RPP8 protein, Arabidopsis Carboxylic Ester Hydrolases PAD4 protein, Arabidopsis
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Eulgem Thomas
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Weigman Victor J
Chang Hur-Song
McDowell John M
Holub Eric B
Glazebrook Jane
Zhu Tong
Dangl Jeffery L
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.040444
Published
2004-06-00
Epub
2004-00-04
Pages
1129-44
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC514145
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · D16978 · United Kingdom
External Links
PubMed source DOI: 10.1104/pp.104.040444 PMC Full Text View journal details
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