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PMID: 15186491 Published · ppublish English Journal Article Validation Study

A survey of ovary-, testis-, and soma-biased gene expression in Drosophila melanogaster adults.

Genome biology ·Vol. 5 ·No. 6 ·2004-00-00 ·Pages R40

Parisi M, Nuttall R, Edwards P, Minor J, Naiman D, Lü J, Doctolero M, Vainer M, Chan C, Malley J, Eastman S, Oliver B

Abstract

Sexual dimorphism results in the formation of two types of individuals with specialized reproductive roles and is most evident in the germ cells and gonads. We have undertaken a global analysis of transcription between the sexes using a 31,464 element FlyGEM microarray to determine what fraction of the genome shows sex-biased expression, what tissues express these genes, the predicted functions of these genes, and where these genes map onto the genome. Females and males (both with and without gonads), dissected testis and ovary, females and males with genetically ablated germlines, and sex-transformed flies were sampled. Using any of a number of criteria, we find extensive sex-biased expression in adults. The majority of cases of sex differential gene expression are attributable to the germ cells. There is also a large class of genes with soma-biased expression. There is little germline-biased expression indicating that nearly all genes with germline expression also show sex-bias. Monte Carlo simulations show that some genes with sex-biased expression are non-randomly distributed in the genome.

MeSH Terms
Animals Blotting, Northern/methods Drosophila melanogaster/genetics Female Gene Expression Profiling/methods Gene Expression Regulation, Developmental/genetics Genes, Insect/genetics,physiology Male Oligonucleotide Array Sequence Analysis/methods Ovary/chemistry,metabolism RNA, Messenger/genetics Research Design Sex Factors Testis/chemistry,metabolism
Chemicals
RNA, Messenger
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Parisi Michael
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. [email protected]
Nuttall Rachel
Edwards Pamela
Minor James
Naiman Daniel
Lü Jining
Doctolero Michael
Vainer Marina
Chan Cathy
Malley James
Eastman Scott
Oliver Brian
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2004-5-6-r40
Email
[email protected]
Published
2004-00-00
Epub
2004-00-01
Pages
R40
Language
English
Region
England
NLM ID
100960660
PMCID
PMC463073
Subset
IM
Grants
NIDDK NIH HHS · Z01 DK015600-10 · United States
External Links
PubMed source DOI: 10.1186/gb-2004-5-6-r40 PMC Full Text View journal details
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