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PMID: 15205677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tob38, a novel essential component in the biogenesis of beta-barrel proteins of mitochondria.

EMBO reports ·Vol. 5 ·No. 7 ·2004-07-00 ·Pages 704-9

Waizenegger T, Habib SJ, Lech M, Mokranjac D, Paschen SA, Hell K, Neupert W, Rapaport D

Abstract

Insertion of beta-barrel proteins into the outer membrane of mitochondria is mediated by the TOB complex. Known constituents of this complex are Tob55 and Mas37. We identified a novel component, Tob38. It is essential for viability of yeast and the function of the TOB complex. Tob38 is exposed on the surface of the mitochondrial outer membrane. It interacts with Mas37 and Tob55 and is associated with Tob55 even in the absence of Mas37. The Tob38-Tob55 core complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane. Depletion of Tob38 results in strongly reduced levels of Tob55 and Mas37 and the residual proteins no longer form a complex. Tob38-depleted mitochondria are deficient in the import of beta-barrel precursor proteins, but not of other outer membrane proteins or proteins of other mitochondrial subcompartments. We conclude that Tob38 has a crucial function in the biogenesis of beta-barrel proteins of mitochondria.

MeSH Terms
Cell Membrane/metabolism Cell Proliferation DNA/metabolism Detergents/pharmacology Dose-Response Relationship, Drug Down-Regulation Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Membrane Proteins/metabolism Mitochondria/metabolism Mitochondrial Proteins/chemistry,metabolism,physiology Open Reading Frames Protein Binding Protein Structure, Secondary Protein Structure, Tertiary Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism,physiology Subcellular Fractions/metabolism Time Factors
Chemicals
Detergents Membrane Proteins Mitochondrial Proteins SAM37 protein, S cerevisiae Saccharomyces cerevisiae Proteins Sam35 protein, S cerevisiae Sam50 protein, S cerevisiae DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Waizenegger Thomas
Institut für Physiologische Chemie der Universität München, Butenandtstrasse 5, Haus B, 81377 Munich, Germany.
Habib Shukry J
Lech Maciej
Mokranjac Dejana
Paschen Stefan A
Hell Kai
Neupert Walter
Rapaport Doron
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7400183
Published
2004-07-00
Epub
2004-00-18
Pages
704-9
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1299094
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.embor.7400183 PMC Full Text View journal details
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