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PMID: 15215315 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth.

Molecular biology of the cell ·Vol. 15 ·No. 9 ·2004-09-00 ·Pages 3977-85

Gao XD, Caviston JP, Tcheperegine SE, Bi E

Abstract

Rho-family GTPases Cdc42p and Rho1p play critical roles in the budding process of the yeast Saccharomyces cerevisiae. However, it is not clear how the functions of these GTPases are coordinated temporally and spatially during this process. Based on its ability to suppress cdc42-Ts mutants when overexpressed, a novel gene PXL1 was identified. Pxl1p resembles mammalian paxillin, which is involved in integrating various signaling events at focal adhesion. Both proteins share amino acid sequence homology and structural organization. When expressed in yeast, chicken paxillin localizes to the sites of polarized growth as Pxl1p does. In addition, the LIM domains in both proteins are the primary determinant for targeting the proteins to the cortical sites in their native cells. These data strongly suggest that Pxl1p is the "ancient paxillin" in yeast. Deletion of PXL1 does not produce any obvious phenotype. However, Pxl1p directly binds to Rho1p-GDP in vitro, and inhibits the growth of rho1-2 and rho1-3 mutants in a dosage-dependent manner. The opposite effects of overexpressed Pxl1p on cdc42 and rho1 mutants suggest that the functions of Cdc42p and Rho1p may be coordinately regulated during budding and that Pxl1p may be involved in this coordination.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Carrier Proteins/chemistry,genetics,metabolism Cell Polarity Chickens Cytoskeletal Proteins/genetics,metabolism Genes, Fungal Molecular Sequence Data Mutation Paxillin Phosphoproteins/genetics,metabolism Protein Binding Recombinant Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid Signal Transduction cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/genetics,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins Cytoskeletal Proteins PXL1 protein, S cerevisiae Paxillin Phosphoproteins Recombinant Proteins Saccharomyces cerevisiae Proteins RHO1 protein, S cerevisiae cdc42 GTP-Binding Protein, Saccharomyces cerevisiae rho GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gao Xiang-Dong
Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104-6058, USA.
Caviston Juliane P
Tcheperegine Serguei E
Bi Erfei
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-01-0079
Published
2004-09-00
Epub
2004-00-23
Pages
3977-85
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC515332
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059216 · United States
NIGMS NIH HHS · GM59216 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-01-0079 PMC Full Text View journal details
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