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PMID: 15225631 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation.

FEBS letters ·Vol. 569 ·No. 1-3 ·2004-07-02 ·Pages 185-90

Travis MA, van der Flier A, Kammerer RA, Mould AP, Sonnenberg A, Humphries MJ

Abstract

Integrin-filamin binding plays an important role in adhesion-mediated control of the actin cytoskeleton. Here, using the interaction between recombinant fragments from the C-terminus of filamin A and the cytoplasmic tail of integrin beta 7 as a model, we report a negative regulatory role for filamin alternative splicing. Splice variant forms of filamin A lacking a 41-amino acid segment interacted more strongly than full-length fragments. In addition, we provide evidence that phosphorylation of the splice variant region is unlikely to represent the mechanism by which binding is reduced.

MeSH Terms

Alternative Splicing/genetics Amino Acid Sequence Amino Acid Substitution Animals Binding Sites COS Cells Chlorocebus aethiops Contractile Proteins/chemistry,genetics,metabolism DNA, Complementary Filamins Integrin beta Chains/chemistry,genetics,metabolism Microfilament Proteins/chemistry,genetics,metabolism Molecular Sequence Data Mutagenesis, Site-Directed Peptide Fragments Phosphorylation Polymerase Chain Reaction/methods Recombinant Proteins/chemistry,metabolism Restriction Mapping Sequence Deletion Transfection

Chemicals

Contractile Proteins DNA, Complementary Filamins Integrin beta Chains Microfilament Proteins Peptide Fragments Recombinant Proteins integrin beta7

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Travis Mark A

School of Biological Sciences, 2.205 Stopford Building, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.

van der Flier Arjan

Kammerer Richard A

Mould A Paul

Sonnenberg Arnoud

Humphries Martin J

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/j.febslet.2004.04.099

Published

2004-07-02

Pages

185-90

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/j.febslet.2004.04.099 View journal details

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Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation.

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