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PMID: 15231715 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex.

Genes & development ·Vol. 18 ·No. 14 ·2004-07-15 ·Pages 1649-54

Ye JZ, Hockemeyer D, Krutchinsky AN, Loayza D, Hooper SM, Chait BT, de Lange T

Abstract

Human telomere length is controlled by a negative feedback loop based on the binding of TRF1 to double-stranded telomeric DNA. The TRF1 complex recruits POT1, a single-stranded telomeric DNA-binding protein necessary for cis-inhibition of telomerase. By mass spectrometry, we have identified a new telomeric protein, which we have named POT1-interacting protein 1 (PIP1). PIP1 bound both POT1 and the TRF1-interacting factor TIN2 and could tether POT1 to the TRF1 complex. Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1.

MeSH Terms
Carrier Proteins/metabolism Cloning, Molecular Fluorescent Antibody Technique HeLa Cells Humans Intracellular Signaling Peptides and Proteins Mass Spectrometry RNA Interference Shelterin Complex Telomere/metabolism,physiology Telomere-Binding Proteins/metabolism Telomeric Repeat Binding Protein 1/metabolism Two-Hybrid System Techniques
Chemicals
Carrier Proteins Intracellular Signaling Peptides and Proteins PAK1IP1 protein, human POT1 protein, human Shelterin Complex TINF2 protein, human Telomere-Binding Proteins Telomeric Repeat Binding Protein 1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ye Jeffrey Zheng-Sheng
Laboratory for Cell Biology and Genetics and Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10021, USA. [email protected]
Hockemeyer Dirk
Krutchinsky Andrew N
Loayza Diego
Hooper Sarah M
Chait Brian T
de Lange Titia
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1215404
Email
[email protected]
Published
2004-07-15
Epub
2004-00-01
Pages
1649-54
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC478187
Subset
IM
Grants
NCRR NIH HHS · P41 RR000862 · United States
NCI NIH HHS · K08 CA093604 · United States
NCI NIH HHS · K08 CA93604 · United States
NCI NIH HHS · T32 CA009673 · United States
NCRR NIH HHS · RR00862 · United States
NCI NIH HHS · T32 CA09673-26A1 · United States
External Links
PubMed source DOI: 10.1101/gad.1215404 PMC Full Text View journal details
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