Home LiteratureArticle Details

PMID: 15232107 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lack of a fusion requirement for development of bone marrow-derived epithelia.

Science (New York, N.Y.) ·Vol. 305 ·No. 5680 ·2004-07-02 ·Pages 90-3

Harris RG, Herzog EL, Bruscia EM, Grove JE, Van Arnam JS, Krause DS

Abstract

Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with beta-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.

MeSH Terms

Animals Bone Marrow Cells/physiology Bone Marrow Transplantation Cell Differentiation Cell Fusion Cobra Cardiotoxin Proteins/pharmacology Elapid Venoms/pharmacology Epithelial Cells/cytology,metabolism Female Green Fluorescent Proteins Hepatocytes/cytology,metabolism Keratinocytes/cytology,metabolism Keratins/analysis Luminescent Proteins/metabolism Male Mice Mice, Transgenic Muscle Cells/cytology Radiation, Ionizing Recombinases/metabolism Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction Stem Cells/cytology,physiology X Chromosome Y Chromosome beta-Galactosidase/biosynthesis,genetics

Chemicals

Cobra Cardiotoxin Proteins Elapid Venoms Luminescent Proteins Recombinases Green Fluorescent Proteins notexin Keratins beta-Galactosidase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Harris Robert G

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.

Herzog Erica L

Bruscia Emanuela M

Grove Joanna E

Van Arnam John S

Krause Diane S

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1098925

Published

2004-07-02

Pages

90-3

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIDDK NIH HHS · DK61846 · United States

NHLBI NIH HHS · HL073742 · United States

NHLBI NIH HHS · HL63357 · United States

NHLBI NIH HHS · T32-HL07778 · United States

Corrections

CommentIn

PMID: 15232079

External Links

PubMed source DOI: 10.1126/science.1098925 View journal details

Analysis Services

Lack of a fusion requirement for development of bone marrow-derived epithelia.

Contact

WeChat Official Account