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PMID: 15240804 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synaptotagmin I synchronizes transmitter release in mouse hippocampal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 24 ·No. 27 ·2004-07-07 ·Pages 6127-32

Nishiki T, Augustine GJ

Abstract

We have asked whether loss of the Ca2+ sensor protein synaptotagmin I influences the total amount of neurotransmitter released after a presynaptic action potential. Hippocampal neurons from synaptotagmin I knock-out mice had a greatly reduced fast synchronous component of glutamate release, as reported previously. However, the amount of glutamate released during the slow asynchronous component increased in these knock-out neurons. As a result of these changes in the kinetics of release, there was no significant difference between wild-type and knock-out neurons in the total amount of transmitter released within 400 msec after a presynaptic stimulus. Fluorescence imaging experiments demonstrated that wild-type and knock-out neurons take up and release similar amounts of FM dye after depolarization, indicating normal amounts of synaptic vesicle trafficking in the knock-out neurons. These results indicate that synaptotagmin I knock-out neurons are fully capable of releasing neurotransmitter, with the increased slow component of release serving to compensate for loss of the fast component. Thus, synaptotagmin I synchronizes the rapid release of neurotransmitters after Ca2+ entry into presynaptic terminals and also appears to suppress the slower, asynchronous form of transmitter release.

MeSH Terms
Animals Calcium-Binding Proteins/genetics,metabolism,physiology Cells, Cultured Endocytosis/physiology Excitatory Postsynaptic Potentials/physiology Exocytosis/physiology Fluorescent Dyes/pharmacokinetics Hippocampus/cytology,metabolism,physiology Membrane Glycoproteins/genetics,metabolism,physiology Mice Mice, Knockout Nerve Tissue Proteins/genetics,metabolism,physiology Neurons/metabolism,physiology Neurotransmitter Agents/metabolism Patch-Clamp Techniques Stimulation, Chemical Synaptic Transmission/physiology Synaptotagmin I Synaptotagmins
Chemicals
Calcium-Binding Proteins Fluorescent Dyes Membrane Glycoproteins Nerve Tissue Proteins Neurotransmitter Agents Synaptotagmin I Syt1 protein, mouse Synaptotagmins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nishiki Tei-ichi
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Augustine George J
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.1563-04.2004
Published
2004-07-07
Pages
6127-32
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6729667
Subset
IM
Grants
NIMH NIH HHS · R01 MH067044 · United States
NINDS NIH HHS · R01 NS021624 · United States
NIMH NIH HHS · MH-67044 · United States
NINDS NIH HHS · NS-21624 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.1563-04.2004 PMC Full Text View journal details
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