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PMID: 15247404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

beta-Amylase induction and the protective role of maltose during temperature shock.

Plant physiology ·Vol. 135 ·No. 3 ·2004-07-00 ·Pages 1674-84

Kaplan F, Guy CL

Abstract

A number of studies have demonstrated beta-amylase induction in response to abiotic stress. In the present work, a temperature response profile in 5 degrees C increments from 45 degrees C to 0 degrees C showed that induction at temperature extremes was specific for two members of the gene family (BMY7 and BMY8). Both members encode proteins that possess apparent transit peptides for chloroplast stromal localization. However, induction was not observed for other key starch degrading enzymes demonstrating a rather specific response to temperature stress for BMY7 and BMY8. Time course experiments for heat shock at 40 degrees C and cold shock at 5 degrees C showed that beta-amylase induction correlated with maltose accumulation. Maltose has the ability, as demonstrated by in vitro assays, to protect proteins, membranes, and the photosynthetic electron transport chain at physiologically relevant concentrations. Therefore, beta-amylase induction and the resultant maltose accumulation may function as a compatible-solute stabilizing factor in the chloroplast stroma in response to acute temperature stress.

MeSH Terms
Base Sequence Chloroplasts/drug effects,enzymology DNA Primers Enzyme Induction Kinetics Maltose/pharmacology Reverse Transcriptase Polymerase Chain Reaction Temperature Thermodynamics beta-Amylase/biosynthesis,genetics
Chemicals
DNA Primers Maltose beta-Amylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaplan Fatma
Plant Molecular and Cellular Biology Program, Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611-0675, USA.
Guy Charles L
References (30)
30 references, click to expand
  1. Orchestrated transcription of key pathways in Arabidopsis by the circadian clock.
    Science. 2000 Dec 15;290(5499):2110-3 PMID: 11118138
  2. Phytohormone-regulated beta-amylase gene expression in rice.
    Plant Mol Biol. 1996 Aug;31(5):975-82 PMID: 8843940
  3. Sequential control of phytochrome-mediated synthesis de novo of β-amylase in the cotyledons of mustard (Sinapis alba L.) seedlings.
    Planta. 1982 Jul;155(2):183-9 PMID: 24271673
  4. Multiple transcription-factor genes are early targets of phytochrome A signaling.
    Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9437-42 PMID: 11481498
  5. Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold.
    Plant Mol Biol. 1998 Dec;38(6):1011-9 PMID: 9869407
  6. Beta-Amylases from Alfalfa (Medicago sativa L.) Roots.
    Plant Physiol. 1982 May;69(5):1096-102 PMID: 16662350
  7. A [beta]-Amylase in Potato Tubers Is Induced by Storage at Low Temperature.
    Plant Physiol. 1997 Feb;113(2):503-510 PMID: 12223621
  8. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
    Plant J. 2002 Aug;31(3):279-92 PMID: 12164808
  9. Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato.
    Plant Mol Biol. 2001 Jul;46(5):627-37 PMID: 11516155
  10. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway.
    Plant Cell. 2002 Aug;14(8):1675-90 PMID: 12172015
  11. Understanding and influencing starch biochemistry.
    Crit Rev Biochem Mol Biol. 2000;35(3):141-96 PMID: 10907795
  12. Maltose is the major form of carbon exported from the chloroplast at night.
    Planta. 2004 Jan;218(3):474-82 PMID: 14566561
  13. Low cost gel analysis.
    Methods Mol Biol. 2000;132:277-88 PMID: 10547841
  14. Living with water stress: evolution of osmolyte systems.
    Science. 1982 Sep 24;217(4566):1214-22 PMID: 7112124
  15. Release and Activity of Bound beta-Amylase in a Germinating Barley Grain.
    Plant Physiol. 1989 Jan;89(1):244-9 PMID: 16666521
  16. Purification of a beta-Amylase that Accumulates in Arabidopsis thaliana Mutants Defective in Starch Metabolism.
    Plant Physiol. 1990 Nov;94(3):1033-9 PMID: 16667793
  17. An Arabidopsis gene encoding a chloroplast-targeted beta-amylase.
    Plant J. 1999 Dec;20(5):519-27 PMID: 10652124
  18. Sucrose-Induced Accumulation of beta-Amylase Occurs Concomitant with the Accumulation of Starch and Sporamin in Leaf-Petiole Cuttings of Sweet Potato.
    Plant Physiol. 1991 Jul;96(3):902-9 PMID: 16668273
  19. Induction of Expression of Genes Coding for Sporamin and beta-Amylase by Polygalacturonic Acid in Leaf-Petiole Cuttings of Sweet Potato.
    Plant Physiol. 1992 Jun;99(2):422-7 PMID: 16668901
  20. The role of amylomaltase in maltose metabolism in the cytosol of photosynthetic cells.
    Planta. 2004 Jan;218(3):466-73 PMID: 14593480
  21. Downregulation of a chloroplast-targeted beta-amylase leads to a starch-excess phenotype in leaves.
    Plant J. 2002 Jun;30(5):581-91 PMID: 12047632
  22. Use of SAGE technology to reveal changes in gene expression in Arabidopsis leaves undergoing cold stress.
    Plant Mol Biol. 2003 Jun;52(3):553-67 PMID: 12956526
  23. A previously unknown maltose transporter essential for starch degradation in leaves.
    Science. 2004 Jan 2;303(5654):87-9 PMID: 14704427
  24. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress.
    Plant Physiol. 2002 Dec;130(4):2129-41 PMID: 12481097
  25. The Role of Pea Chloroplast [alpha]-Glucosidase in Transitory Starch Degradation.
    Plant Physiol. 1995 May;108(1):211-217 PMID: 12228465
  26. Sugar-inducible expression of a gene for beta-amylase in Arabidopsis thaliana.
    Plant Physiol. 1995 Mar;107(3):895-904 PMID: 7716246
  27. Multiple pathways for protein transport into or across the thylakoid membrane.
    EMBO J. 1993 Nov;12(11):4105-14 PMID: 8223427
  28. Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts.
    Plant Physiol. 1986 Dec;82(4):1119-21 PMID: 16665144
  29. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray.
    Plant Cell. 2001 Jan;13(1):61-72 PMID: 11158529
  30. Purification and Characterization of Pea Epicotyl beta-Amylase.
    Plant Physiol. 1990 Mar;92(3):615-21 PMID: 16667324
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.040808
Published
2004-07-00
Epub
2004-00-09
Pages
1674-84
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC519081
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.104.040808 PMC Full Text View journal details
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