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PMID: 15257295 Published · ppublish English Journal Article

Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs.

The EMBO journal ·Vol. 23 ·No. 15 ·2004-08-04 ·Pages 3092-102

Lal A, Mazan-Mamczarz K, Kawai T, Yang X, Martindale JL, Gorospe M

Abstract

RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been systematically studied. Here, using common target RNAs encoding p21 and cyclin D1, we provide evidence that HuR and AUF1 can bind target transcripts on both distinct, nonoverlapping sites, and on common sites in a competitive fashion. In the nucleus, both proteins were found together within stable ribonucleoprotein complexes; in the cytoplasm, HuR and AUF1 were found to bind to target mRNAs individually, HuR colocalizing with the translational apparatus and AUF1 with the exosome. Our results indicate that the composition and fate (stability, translation) of HuR- and/or AUF1-containing ribonucleoprotein complexes depend on the target mRNA of interest, RNA-binding protein abundance, stress condition, and subcellular compartment.

MeSH Terms
Antigens, Surface/genetics,metabolism Cell Cycle Proteins/genetics Cyclin D1/genetics Cyclin-Dependent Kinase Inhibitor p21 ELAV Proteins ELAV-Like Protein 1 Gene Expression Regulation/radiation effects HeLa Cells Heterogeneous Nuclear Ribonucleoprotein D0 Heterogeneous-Nuclear Ribonucleoprotein D/genetics,metabolism Humans RNA Stability RNA, Messenger/chemical synthesis,genetics,metabolism RNA, Small Interfering/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Substrate Specificity Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
Antigens, Surface CDKN1A protein, human Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor p21 ELAV Proteins ELAV-Like Protein 1 ELAVL1 protein, human HNRNPD protein, human Heterogeneous Nuclear Ribonucleoprotein D0 Heterogeneous-Nuclear Ribonucleoprotein D RNA, Messenger RNA, Small Interfering RNA-Binding Proteins Cyclin D1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lal Ashish
Laboratory of Cellular and Molecular Biology, National Institute on Aging-IRP, National Institutes of Health, Baltimore, MD 21224, USA.
Mazan-Mamczarz Krystyna
Kawai Tomoko
Yang Xiaoling
Martindale Jennifer L
Gorospe Myriam
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600305
Published
2004-08-04
Epub
2004-00-15
Pages
3092-102
Language
English
Region
England
NLM ID
8208664
PMCID
PMC514922
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600305 PMC Full Text View journal details
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