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PMID: 15270081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Administration of Hsp70 in vivo inhibits motor and sensory neuron degeneration.

Cell stress & chaperones ·Vol. 9 ·No. 1 ·2004-03-00 ·Pages 88-98

Tidwell JL, Houenou LJ, Tytell M

Abstract

The induction of heat shock proteins (Hsps) serves not only as a marker for cellular stress but also as a promoter of cell survival, which is especially important in the nervous system. We examined the regulation of the constitutive and stress-induced 70-kD Hsps (Hsc70 and Hsp70, respectively) after sciatic nerve (SN) axotomy in the neonatal mouse. Additionally, the prevention of axotomy-induced SN cell death by administration of several preparations of exogenous Hsc70 and Hsp70 was tested. Immunohistochemistry and Western blot analyses showed that endogenous levels of Hsc70 and Hsp70 did not increase significantly in lumbar motor neurons or dorsal root ganglion sensory neurons up to 24 hours after axotomy. When a variety of Hsc70 and Hsp70 preparations at doses ranging from 5 to 75 microg were applied to the SN stump after axotomy, the survival of both motor and sensory neurons was significantly improved. Thus, it appears that motor and sensory neurons in the neonatal mouse do not initiate a typical Hsp70 response after traumatic injury and that administration of exogenous Hsc/Hsp70 can remedy that deficit and reduce the subsequent loss of neurons by apoptosis.

MeSH Terms
Absorbable Implants Animals Animals, Newborn Axotomy Blotting, Western Cell Survival/drug effects Ganglia, Spinal/drug effects,metabolism,pathology HSC70 Heat-Shock Proteins HSP70 Heat-Shock Proteins/administration & dosage,metabolism,pharmacology Immunohistochemistry Mice Mice, Inbred BALB C Motor Neurons/drug effects,metabolism,pathology Nerve Degeneration/drug therapy,metabolism Neurons, Afferent/drug effects,metabolism,pathology Recombinant Proteins/metabolism,pharmacology Sciatic Nerve/drug effects,metabolism,pathology
Chemicals
HSC70 Heat-Shock Proteins HSP70 Heat-Shock Proteins Hspa8 protein, mouse Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tidwell J Lille
Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1010, USA.
Houenou Lucien J
Tytell Michael
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Article Info
Journal
Cell stress & chaperones
Abbr.
Cell Stress Chaperones
ISSN
1355-8145
DOI
10.1379/csc-9r.1
Published
2004-03-00
Pages
88-98
Language
English
Region
Netherlands
NLM ID
9610925
PMCID
PMC1065310
Subset
IM
External Links
PubMed source DOI: 10.1379/csc-9r.1 PMC Full Text View journal details
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