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PMID: 1527166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tissue distribution and subcellular localization of mammalian myosin I.

The Journal of cell biology ·Vol. 119 ·No. 1 ·1992-10-00 ·Pages 163-70

Wagner MC, Barylko B, Albanesi JP

Abstract

Myosin I, a nonfilamentous single-headed actin-activated ATPase, has recently been purified from mammalian tissue (Barylko, B., M. C. Wagner, O. Reizes, and J. P. Albanesi. 1992. Proc. Natl. Acad. Sci. USA. 89:490-494). To investigate the distribution of this enzyme in cells and tissues mAbs were generated against myosin I purified from bovine adrenal gland. Eight antibodies were characterized, five of them (M4-M8) recognize epitope(s) on the catalytic "head" portion of myosin I while the other three (M1-M3) react with the "tail" domain. Immunoblot analysis using antiadrenal myosin I antibody M2 demonstrates the widespread distribution of the enzyme in mammalian tissues. Myosin I was immunolocalized in several cell types including bovine kidney (MDBK), rat kidney (NRK), rat brain, rat phaeochromocytoma (PC12), fibroblast (Swiss 3T3), and CHO cells. In all cases, myosin I was concentrated at the cell periphery. The most intense labeling was observed in regions of the cell usually associated with motile activity (i.e., filopodia, lamellipodia and growth cones). These results are consistent with earlier observations on protozoan myosin I that suggest a motile role for the enzyme at the plasma membrane.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Cell Line Cell Membrane/chemistry Cytoplasm/chemistry Fluorescent Antibody Technique Hybridomas Kidney/chemistry Myosins/analysis,immunology Neurons/chemistry Rats
Chemicals
Antibodies, Monoclonal Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wagner M C
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Barylko B
Albanesi J P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.1.163
Published
1992-10-00
Pages
163-70
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289625
Subset
IM
Grants
NIGMS NIH HHS · GM38567 · United States
External Links
PubMed source DOI: 10.1083/jcb.119.1.163 PMC Full Text View journal details
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