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PMID: 15282548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Visualization of RNA-protein interactions in living cells: FMRP and IMP1 interact on mRNAs.

The EMBO journal ·Vol. 23 ·No. 16 ·2004-08-18 ·Pages 3346-55

Rackham O, Brown CM

Abstract

Protein expression depends significantly on the stability, translation efficiency and localization of mRNA. These qualities are largely dictated by the RNA-binding proteins associated with an mRNA. Here, we report a method to visualize and localize RNA-protein interactions in living mammalian cells. Using this method, we found that the fragile X mental retardation protein (FMRP) isoform 18 and the human zipcode-binding protein 1 ortholog IMP1, an RNA transport factor, were present on common mRNAs. These interactions occurred predominantly in the cytoplasm, in granular structures. In addition, FMRP and IMP1 interacted independently of RNA. Tethering of FMRP to an mRNA caused IMP1 to be recruited to the same mRNA and resulted in granule formation. The intimate association of FMRP and IMP1 suggests a link between mRNA transport and translational repression in mammalian cells.

MeSH Terms
Actins/metabolism Animals COS Cells Chlorocebus aethiops Cytoskeletal Proteins Fragile X Mental Retardation Protein Humans Multiprotein Complexes/chemistry,metabolism Nerve Tissue Proteins/metabolism Protein Binding RNA, Messenger/metabolism RNA-Binding Proteins/metabolism
Chemicals
Actins Cytoskeletal Proteins FMR1 protein, human IGF2BP1 protein, human Multiprotein Complexes Nerve Tissue Proteins RNA, Messenger RNA-Binding Proteins STAU1 protein, human Fragile X Mental Retardation Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rackham Oliver
Biochemistry Department, University of Otago, Dunedin, New Zealand.
Brown Chris M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600341
Published
2004-08-18
Epub
2004-00-29
Pages
3346-55
Language
English
Region
England
NLM ID
8208664
PMCID
PMC514514
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600341 PMC Full Text View journal details
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