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PMID: 15284342 Published · ppublish English Comparative Study Journal Article

Electroneutral ammonium transport by basolateral rhesus B glycoprotein.

The Journal of physiology ·Vol. 559 ·No. Pt 3 ·2004-09-15 ·Pages 751-9

Ludewig U

Abstract

The liver and kidney are important tissues for ammonium (NH4+/NH3) metabolism and excretion. The rhesus B glycoprotein (RhBG) is a membrane protein expressed in liver and kidney with similarity to NH4+ transporters found in microorganisms, plants and animals. In the kidney, RhBG is predominantly localized to basolateral membranes of distal tubule epithelia, including connecting tubules and collecting ducts. These epithelia display mainly electroneutral ammonium transport, in contrast to other tubular sites, where net NH4+ transport occurs. In accordance with its localization, human RhBG mediates saturable, electroneutral transport of the ammonium analogue methylammonium when heterologously expressed in Xenopus oocytes. Uptake of methylammonium saturates with a Km = 2.6 mm. Methylammonium uptake is inhibited by ammonium and this inhibition saturates with a Ki approximately 3 mm. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+ -H+ exchanger.

MeSH Terms
Animals Antiporters/genetics,metabolism Biological Transport, Active/physiology Female Glycoproteins/genetics,metabolism Humans Membrane Transport Proteins/genetics,metabolism Methylamines/metabolism Oocytes/metabolism Quaternary Ammonium Compounds/metabolism Xenopus
Chemicals
Antiporters Glycoproteins Membrane Transport Proteins Methylamines Quaternary Ammonium Compounds RHBG protein, human methylamine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ludewig Uwe
Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Germany. [email protected]
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.2004.067728
Email
[email protected]
Published
2004-09-15
Epub
2004-00-29
Pages
751-9
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1665183
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.2004.067728 PMC Full Text View journal details
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