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PMID: 15289464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells.

Genes & development ·Vol. 18 ·No. 16 ·2004-08-15 ·Pages 1970-5

Chiu CH, Lin WD, Huang SY, Lee YH

Abstract

CCAAT/enhancer-binding proteins, C/EBPalpha and C/EBPbeta, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPalpha with C/EBPbeta, generating the beta/beta alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). In this study, beta/beta mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of beta/beta mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The beta/beta allele exerted its effect through the elevated expression of the G protein alpha stimulatory subunit (Galphas) in WAT. Galphas, when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of beta/beta mice, and effectively diminished the stored lipid pool.

MeSH Terms
Alleles Animals CCAAT-Enhancer-Binding Protein-alpha/genetics CCAAT-Enhancer-Binding Protein-beta/genetics Energy Metabolism GTP-Binding Proteins/metabolism Mice Mitochondria/metabolism
Chemicals
CCAAT-Enhancer-Binding Protein-alpha CCAAT-Enhancer-Binding Protein-beta GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chiu Chih-Hsien
Laboratory of Molecular Pathology, Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan.
Lin Wen-Der
Huang Shao-Yong
Lee Ying-Hue
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1213104
Published
2004-08-15
Epub
2004-00-02
Pages
1970-5
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC514177
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1213104 PMC Full Text View journal details
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