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PMID: 15300440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco.

Planta ·Vol. 220 ·No. 2 ·2004-12-00 ·Pages 262-70

Zhang H, Huang Z, Xie B, Chen Q, Tian X, Zhang X, Zhang H, Lu X, Huang D, Huang R

Abstract

Ethylene responsive factors (ERFs) are important plant-specific transcription factors, some of which have been demonstrated to interact with the ethylene-responsive GCC box and the dehydration-responsive element (DRE); however, data on the roles of ERF proteins in connection with various signaling pathways are limited. In this research, we used the GCC box, an essential cis-acting element responsive to ethylene and methyl jasmonate (MeJA), as bait in a yeast one-hybrid system to isolate transcription factors from tomato (Lycopersicon esculentum Mill.). One of the cDNAs, which was designated Jasmonate and Ethylene Response Factor 1 (JERF1), encodes an ERF protein, containing a conserved ERF DNA-binding motif and functioning as a transcriptional activator in yeast through targeting to the nucleus in onion (Allium cepa L.) epidermal cells. Biochemical analysis revealed that JERF1 bound not only to the GCC box but also to the DRE sequence. Expression of the JERF1 gene in tomato was induced by ethylene, MeJA, abscisic acid (ABA) and salt treatment, indicating that JERF1 might act as a connector among different signal transduction pathways. Further research with transgenic JERF1 tobacco (Nicotiana tabacum L.) plants indicated that overexpressing JERF1 activated expression of GCC box-containing genes such as osmotin, GLA, Prb-1b and CHN50 under normal growth conditions, and subsequently resulted in enhanced tolerance to salt stress, suggesting that JERF1 modulates osmotic tolerance by activation of downstream gene expression through interaction with the GCC box or DRE.

MeSH Terms
Abscisic Acid/pharmacology Acetates/pharmacology Amino Acid Sequence Base Sequence Cyclopentanes/pharmacology DNA, Complementary DNA, Plant Ethylenes/pharmacology Gene Expression Regulation, Plant/physiology Lycopersicon esculentum/physiology Molecular Sequence Data Oxylipins Plant Growth Regulators/pharmacology Plants, Genetically Modified Regulatory Sequences, Nucleic Acid Signal Transduction Sodium Chloride/pharmacology Tobacco/drug effects,genetics,metabolism Transcription Factors/genetics,physiology
Chemicals
Acetates Cyclopentanes DNA, Complementary DNA, Plant Ethylenes Oxylipins Plant Growth Regulators Transcription Factors Sodium Chloride Abscisic Acid methyl jasmonate ethylene
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Zhang Haiwen
The National Plant Gene Research Center (Beijing), Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081 Beijing, China.
Huang Zejun
Xie Bingyan
Chen Qi
Tian Xin
Zhang Xiulin
Zhang Hongbo
Lu Xiangyang
Huang Dafang
Huang Rongfeng
References (49)
49 references, click to expand
  1. New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element.
    FEBS Lett. 2003 Aug 28;550(1-3):149-54 PMID: 12935902
  2. Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2992-7 PMID: 12606727
  3. Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana.
    Cell. 1998 Jul 24;94(2):261-71 PMID: 9695954
  4. Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor.
    Cell. 2003 Dec 12;115(6):667-77 PMID: 14675532
  5. Jasmonate and salicylate as global signals for defense gene expression.
    Curr Opin Plant Biol. 1998 Oct;1(5):404-11 PMID: 10066616
  6. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11655-60 PMID: 11027363
  7. Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana.
    Genetics. 2000 Jan;154(1):421-36 PMID: 10629000
  8. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.
    Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2122-7 PMID: 9122158
  9. Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco.
    Plant Cell. 2001 May;13(5):1035-46 PMID: 11340180
  10. Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis.
    Plant Cell. 1998 Dec;10 (12 ):2103-13 PMID: 9836748
  11. Tissue-specific activation of the osmotin gene by ABA, C2H4 and NaCl involves the same promoter region.
    Plant Mol Biol. 1997 Jun;34(3):393-402 PMID: 9225851
  12. Interacting signal pathways control defense gene expression in Arabidopsis in response to cell wall-degrading enzymes from Erwinia carotovora.
    Mol Plant Microbe Interact. 2000 Apr;13(4):430-8 PMID: 10755306
  13. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein.
    Proc Natl Acad Sci U S A. 1997 May 27;94(11):5961-6 PMID: 9159183
  14. Transcriptional control of plant genes responsive to pathogens.
    Curr Opin Plant Biol. 1998 Aug;1(4):311-5 PMID: 10066598
  15. Rapid transcript accumulation of pathogenesis-related genes during an incompatible interaction in bacterial speck disease-resistant tomato plants.
    Plant Mol Biol. 1999 Jun;40(3):455-65 PMID: 10437829
  16. A basic-type PR-1 promoter directs ethylene responsiveness, vascular and abscission zone-specific expression.
    Plant J. 1993 Aug;4(2):225-34 PMID: 8220480
  17. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression.
    Biochem Biophys Res Commun. 2002 Jan 25;290(3):998-1009 PMID: 11798174
  18. Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1820-4 PMID: 2000389
  19. Gene structure and expression of a tobacco endochitinase gene in suspension-cultured tobacco cells.
    Plant Mol Biol. 1991 Jan;16(1):1-10 PMID: 1888889
  20. A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2.
    EMBO J. 1999 Aug 16;18(16):4455-63 PMID: 10449411
  21. BIOSYNTHESIS AND ACTION OF JASMONATES IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:355-381 PMID: 15012267
  22. A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol. 2003 Jun;132(2):1020-32 PMID: 12805630
  23. A role for jasmonate in pathogen defense of Arabidopsis.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7209-14 PMID: 9618564
  24. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element.
    Plant Cell. 1995 Feb;7(2):173-82 PMID: 7756828
  25. A GCC element and a G-box motif participate in ethylene-induced expression of the PRB-1b gene.
    Plant Mol Biol. 1995 Apr;28(1):145-53 PMID: 7787179
  26. The Arabidopsis male-sterile mutant, opr3, lacks the 12-oxophytodienoic acid reductase required for jasmonate synthesis.
    Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10625-30 PMID: 10973494
  27. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit.
    Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1035-40 PMID: 9023378
  28. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes.
    EMBO J. 1997 Jun 2;16(11):3207-18 PMID: 9214637
  29. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.
    Genes Dev. 1998 Dec 1;12(23):3703-14 PMID: 9851977
  30. Transcriptional activation of plant defense genes.
    Curr Opin Genet Dev. 1996 Oct;6(5):624-30 PMID: 8939722
  31. Identification of an ethylene-responsive region in the promoter of a tobacco class I chitinase gene.
    Plant Mol Biol. 1995 Mar;27(5):923-32 PMID: 7766882
  32. Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain.
    Plant J. 1997 Mar;11(3):513-23 PMID: 9107039
  33. ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense.
    Plant Cell. 2003 Jan;15(1):165-78 PMID: 12509529
  34. EIN3-dependent regulation of plant ethylene hormone signaling by two arabidopsis F box proteins: EBF1 and EBF2.
    Cell. 2003 Dec 12;115(6):679-89 PMID: 14675533
  35. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression.
    Plant Cell. 2000 Mar;12(3):393-404 PMID: 10715325
  36. Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the Pto kinase.
    Plant Cell. 2000 May;12(5):771-86 PMID: 10810149
  37. Interactions between abscisic acid and ethylene signaling cascades.
    Plant Cell. 2000 Jul;12(7):1103-15 PMID: 10899977
  38. The arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway.
    Plant Cell. 2000 Jul;12(7):1041-61 PMID: 10899973
  39. Tomato transcription factors pti4, pti5, and pti6 activate defense responses when expressed in Arabidopsis.
    Plant Cell. 2002 Apr;14(4):817-31 PMID: 11971137
  40. The jasmonate-inducible AP2/ERF-domain transcription factor ORCA3 activates gene expression via interaction with a jasmonate-responsive promoter element.
    Plant J. 2001 Jan;25(1):43-53 PMID: 11169181
  41. Cell signaling during cold, drought, and salt stress.
    Plant Cell. 2002;14 Suppl:S165-83 PMID: 12045276
  42. In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves.
    Plant J. 2000 Jun;22(6):543-51 PMID: 10886774
  43. Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory.
    Curr Opin Plant Biol. 2003 Aug;6(4):372-8 PMID: 12873533
  44. Pseudomonas syringae pv tomato induces the expression of tomato EREBP-like genes pti4 and pti5 independent of ethylene, salicylate and jasmonate
    Plant J. 1999 Nov;20(4):475-83 PMID: 10607299
  45. The ethylene signaling pathway: new insights.
    Curr Opin Plant Biol. 2004 Feb;7(1):40-9 PMID: 14732440
  46. The tomato transcription factor Pti4 regulates defense-related gene expression via GCC box and non-GCC box cis elements.
    Plant Cell. 2003 Dec;15(12):3033-50 PMID: 14630974
  47. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
    Plant Cell. 1998 Aug;10(8):1391-406 PMID: 9707537
  48. Plant Defense Genes Are Synergistically Induced by Ethylene and Methyl Jasmonate.
    Plant Cell. 1994 Aug;6(8):1077-1085 PMID: 12244267
  49. Glyoxalase I from Brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress.
    Plant J. 1999 Feb;17(4):385-95 PMID: 10205896
Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-004-1347-x
Published
2004-12-00
Epub
2004-00-05
Pages
262-70
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
Databases
GENBANK
AB008104, AB008105, AF058827, AF076277, AF093743, AY044235, D38123, D38126, M60402, U89255, U89256, X51599, X66942, X95308
External Links
PubMed source DOI: 10.1007/s00425-004-1347-x View journal details
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