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PMID: 1530574 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Uptake of polyamines by human endothelial cells. Characterization and lack of effect of agonists of endothelial function.

The Biochemical journal ·Vol. 286 ( Pt 2) ·1992-09-01 ·Pages 413-7

Morgan DM

Abstract

Uptake of polyamines by confluent monolayers of human umbilical-vein endothelial cells (HUVECs) was found to be time-, temperature- and concentration-dependent, energy-requiring, and saturable. Kinetic constants were putrescine Kt 3 +/- 1 microM, Vmax. 15 +/- 7 pmol/h per microgram of protein; spermidine, 0.7 +/- 0.2, 12 +/- 3; spermine, 1 +/- 0.7, 11 +/- 4. Putrescine uptake was inhibited by spermine or spermidine, whereas uptake of spermine or spermidine was not inhibited by 20 microM-putrescine. These data suggest the existence of two carriers, one shared by spermine and spermidine, and one capable of transporting all three polyamines. Pretreatment of HUVECs with thrombin (less than or equal to 10 units/ml; 1 h), bradykinin (less than or equal to 10 microM; 1 h), interleukin-1 (less than or equal to 100 units/ml; 2 h) or phorbol 12-myristate 13-acetate (less than or equal to 1.0 microM; 1 h), all known agonists of endothelial function, had no significant effect on polyamine uptake. These responses may be of importance in angiogenesis and wound healing, and could have pharmacological significance, for there is a growing interest in the use of polyamines or polyamine analogues as therapeutic agents.

MeSH Terms
2,4-Dinitrophenol Bradykinin/pharmacology Cells, Cultured Chloromercuribenzoates/pharmacology Dinitrophenols/pharmacology Dithiothreitol/pharmacology Endothelium, Vascular/cytology,drug effects,metabolism Ethylmaleimide/pharmacology Humans Interleukin-1/pharmacology Kinetics Polyamines/metabolism Tetradecanoylphorbol Acetate/pharmacology Thrombin/pharmacology p-Chloromercuribenzoic Acid
Chemicals
Chloromercuribenzoates Dinitrophenols Interleukin-1 Polyamines p-Chloromercuribenzoic Acid Thrombin Tetradecanoylphorbol Acetate Ethylmaleimide 2,4-Dinitrophenol Bradykinin Dithiothreitol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Morgan D M
Vascular Biology Research Centre, King's College London, U.K.
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2860413
Published
1992-09-01
Pages
413-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132914
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2860413 PMC Full Text View journal details
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