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PMID: 1530591 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation.

The Biochemical journal ·Vol. 286 ( Pt 2) ·1992-09-01 ·Pages 607-11

Reznick AZ, Cross CE, Hu ML, Suzuki YJ, Khwaja S, Safadi A, Motchnik PA, Packer L, Halliwell B

Abstract

Exposure of human plasma to gas-phase (but not to whole) cigarette smoke (CS) produces oxidative damage to lipids [Frei, Forte, Ames & Cross (1991) Biochem. J. 277, 133-138], which is prevented by ascorbic acid. The ability of CS to induce protein damage was measured by the carbonyl assay and by loss of enzyme activity and protein -SH groups. Both whole and gas-phase CS caused formation of carbonyls in human plasma, which was partially inhibited by GSH but not by ascorbic acid or metal-ion-chelating agents. Isolated albumin exposed to CS showed much faster carbonyl formation (per unit protein) than did whole plasma; damage to isolated albumin was partially prevented by chelating agents. Isolated creatine kinase (CK) lost activity upon exposure to CS much faster than did CK in plasma. Direct addition to plasma of mixtures of some or all of the aldehydes reported to be present in CS caused protein carbonyl formation and inactivation of CK, but neither occurred to the extent produced by CS exposure.

MeSH Terms
Adult Antioxidants/pharmacology Blood Proteins/drug effects,metabolism Deferoxamine/chemistry Free Radicals Glutathione/chemistry Humans Male Middle Aged Oxidation-Reduction Tobacco Smoke Pollution/adverse effects
Chemicals
Antioxidants Blood Proteins Free Radicals Tobacco Smoke Pollution Glutathione Deferoxamine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Reznick A Z
Membrane Bioenergetics Group, University of California, Berkeley 94720.
Cross C E
Hu M L
Suzuki Y J
Khwaja S
Safadi A
Motchnik P A
Packer L
Halliwell B
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2860607
Published
1992-09-01
Pages
607-11
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132941
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2860607 PMC Full Text View journal details
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