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PMID: 1531630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two different types of lipid moieties are present in glycophosphoinositol-anchored membrane proteins of Saccharomyces cerevisiae.

The EMBO journal ·Vol. 11 ·No. 2 ·1992-02-00 ·Pages 457-66

Conzelmann A, Puoti A, Lester RL, Desponds C

Abstract

Numerous glycoproteins of Saccharomyces cerevisiae are anchored in the lipid bilayer by a glycophosphatidylinositol (GPI) anchor. Mild alkaline hydrolysis reveals that the lipid components of these anchors are heterogeneous in that both base-sensitive and base-resistant lipid moieties can be found on most proteins. The relative abundance of base-resistant lipid moieties is different for different proteins. Strong alkaline or acid hydrolysis of the mild base-resistant lipid component liberates C18-phytosphingosine indicating the presence of a ceramide. Two lines of evidence suggest that proteins are first attached to a base-sensitive GPI anchor, the lipid moiety of which subsequently gets exchanged for a base-resistant ceramide: (i) an early glycolipid intermediate of GPI biosynthesis only contains base-sensitive lipid moieties; (ii) after a pulse with [3H]myo-inositol the relative abundance of base-sensitive GPI anchors decreases significantly during chase. This decrease does not take place if GPI-anchored proteins are retained in the ER.

MeSH Terms
Chromatography, Affinity Chromatography, High Pressure Liquid Glycolipids/analysis,metabolism Glycosylphosphatidylinositols Inositol/metabolism Kinetics Lipid Bilayers Membrane Proteins/isolation & purification,metabolism Molecular Weight Palmitic Acid Palmitic Acids/metabolism Phosphatidylinositols/analysis,metabolism Radioisotope Dilution Technique Saccharomyces cerevisiae/metabolism Tritium
Chemicals
Glycolipids Glycosylphosphatidylinositols Lipid Bilayers Membrane Proteins Palmitic Acids Phosphatidylinositols Tritium Palmitic Acid Inositol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Conzelmann A
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Puoti A
Lester R L
Desponds C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-02-00
Pages
457-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556475
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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