Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 epsilon.

Science (New York, N.Y.) ·Vol. 255 ·No. 5040 ·1992-01-03 ·Pages 79-82

Letourneur F, Klausner RD

Abstract

The multichain T cell antigen receptor functions by interacting with and activating one or more nonreceptor tyrosine kinases. The cytoplasmic tail of the zeta chain can activate T cells independently of the rest of the receptor complex. The function of the remaining invariant CD3 chains remains unknown. A 22-amino acid region of the cytoplasmic tail of CD3 epsilon was also able to independently activate T cells. Stimulation of T cells by means of the cytoplasmic tails of either zeta or CD3 epsilon resulted in quantitatively distinct patterns of tyrosine phosphorylation, suggesting activation of different biochemical pathways.

MeSH Terms

Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte/genetics,metabolism CD3 Complex Cell Line Cell Membrane/immunology,metabolism Chimera Clone Cells Humans Interleukin-2/biosynthesis Kinetics Lymphocyte Activation Macromolecular Substances Mice Molecular Sequence Data Mutagenesis, Site-Directed Phosphorylation Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics,metabolism Receptors, Antigen, T-Cell/genetics,metabolism Receptors, Interleukin-2/genetics,metabolism T-Lymphocytes/enzymology,immunology Transfection

Chemicals

Antigens, Differentiation, T-Lymphocyte CD3 Complex Interleukin-2 Macromolecular Substances Receptors, Antigen, T-Cell Receptors, Interleukin-2 Protein-Tyrosine Kinases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Letourneur F

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

Klausner R D

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1532456

Published

1992-01-03

Pages

79-82

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1532456 View journal details

Analysis Services

Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 epsilon.

Contact

WeChat Official Account