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PMID: 1534750 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

An extra copy of nimEcyclinB elevates pre-MPF levels and partially suppresses mutation of nimTcdc25 in Aspergillus nidulans.

The EMBO journal ·Vol. 11 ·No. 6 ·1992-06-00 ·Pages 2139-49

O'Connell MJ, Osmani AH, Morris NR, Osmani SA

Abstract

Previous work has shown that nimA encodes a cell cycle regulated protein kinase that is required along with the p34cdc2 histone H1 kinase (MPF) for mitosis in Aspergillus nidulans. We have now identified two other gene products required for mitosis in A.nidulans. nimT encodes a protein similar to the fission yeast cdc25 tyrosine phosphatase and is required for the conversion of pre-MPF to MPF and nimE encodes a B-type cyclin which is a subunit of MPF. A new genetic interaction between nimEcyclinB and nimTcdc25 type genes is reported. Increased copy number of nimEcyclinB can suppress mutation of nimTcdc25 and also lead to increased accumulation of tyrosine phosphorylated p34cdc2 (pre-MPF). This biochemical observation suggests an explanation for the genetic complementation. If nimEcyclinB recruits p34cdc2 for tyrosine phosphorylation to form pre-MPF it follows that increased expression of nimEcyclinB would increase the level of pre-MPF. The increased level of pre-MPF generated may then allow the mutant nimTcdc25 protein to convert enough pre-MPF to MPF and thus permit some mitotic progression. We also demonstrate that correct cell cycle regulation by the p34cdc2 protein kinase pathway is essential for correct developmental progression in A.nidulans.

MeSH Terms
Amino Acid Sequence Aspergillus nidulans/cytology,enzymology,genetics Base Sequence CDC2 Protein Kinase/genetics Cell Cycle/genetics Chromosomes, Fungal Cloning, Molecular Cosmids Cyclins/genetics,metabolism Fungal Proteins/genetics,metabolism Gene Library Genes, Fungal Maturation-Promoting Factor/genetics Mitosis/genetics Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Protein Kinases/genetics Protein Precursors/genetics Sequence Homology, Nucleic Acid Suppression, Genetic
Chemicals
Cyclins Fungal Proteins Oligodeoxyribonucleotides Protein Precursors maturation-promoting factor precursor NIME protein, Emericella nidulans Protein Kinases CDC2 Protein Kinase Maturation-Promoting Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Connell M J
Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
Osmani A H
Morris N R
Osmani S A
References (42)
42 references, click to expand
  1. Activation of the nimA protein kinase plays a unique role during mitosis that cannot be bypassed by absence of the bimE checkpoint.
    EMBO J. 1991 Sep;10(9):2669-79 PMID: 1868838
  2. Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner.
    EMBO J. 1991 May;10(5):1255-63 PMID: 1850698
  3. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
    Cell. 1991 Oct 4;67(1):197-211 PMID: 1913817
  4. Parallel activation of the NIMA and p34cdc2 cell cycle-regulated protein kinases is required to initiate mitosis in A. nidulans.
    Cell. 1991 Oct 18;67(2):283-91 PMID: 1913824
  5. Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans.
    Nucleic Acids Res. 1991 Jun 11;19(11):3105-9 PMID: 2057366
  6. Molecular genetics of Aspergillus development.
    Annu Rev Genet. 1990;24:5-36 PMID: 2088177
  7. Universal control mechanism regulating onset of M-phase.
    Nature. 1990 Apr 5;344(6266):503-8 PMID: 2138713
  8. The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
    EMBO J. 1990 Sep;9(9):2865-75 PMID: 2143983
  9. Cyclin activation of p34cdc2.
    Cell. 1990 Nov 30;63(5):1013-24 PMID: 2147872
  10. Driving the cell cycle: M phase kinase, its partners, and substrates.
    Cell. 1990 Jun 1;61(5):743-52 PMID: 2160858
  11. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
    Proc Natl Acad Sci U S A. 1990 Jul;87(13):5139-43 PMID: 2195549
  12. Regulation of mitosis by cyclic accumulation of p80cdc25 mitotic inducer in fission yeast.
    Nature. 1990 Apr 5;344(6266):549-52 PMID: 2320127
  13. Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
    Cell. 1989 Jul 14;58(1):181-91 PMID: 2473838
  14. The role of cyclin synthesis, modification and destruction in the control of cell division.
    J Cell Sci Suppl. 1989;12:77-97 PMID: 2534558
  15. Conservation of mitotic controls in fission and budding yeasts.
    Cell. 1989 Apr 21;57(2):295-303 PMID: 2649252
  16. Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes.
    Gene. 1989 Jun 30;79(1):119-30 PMID: 2673931
  17. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
    Nature. 1989 Nov 2;342(6245):39-45 PMID: 2682257
  18. Genetic control of cell division patterns in the Drosophila embryo.
    Cell. 1989 Apr 7;57(1):177-87 PMID: 2702688
  19. Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules.
    EMBO J. 1988 Aug;7(8):2321-7 PMID: 2847913
  20. Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
    Cell. 1987 May 22;49(4):559-67 PMID: 3032459
  21. Spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene.
    Cell. 1988 Jan 29;52(2):241-51 PMID: 3277718
  22. Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulans.
    J Cell Biol. 1987 Jun;104(6):1495-504 PMID: 3294854
  23. Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans.
    Gene. 1987;61(3):385-99 PMID: 3328733
  24. Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase.
    Cell. 1988 Apr 22;53(2):237-44 PMID: 3359487
  25. Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase.
    EMBO J. 1987 Nov;6(11):3441-7 PMID: 3428262
  26. cdc25+ functions as an inducer in the mitotic control of fission yeast.
    Cell. 1986 Apr 11;45(1):145-53 PMID: 3955656
  27. S-phase, G2, and nuclear division mutants of Aspergillus nidulans.
    J Bacteriol. 1984 Jul;159(1):114-9 PMID: 6376468
  28. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  29. Clues to action of cdc25 protein.
    Nature. 1991 May 16;351(6323):194 PMID: 1645849
  30. The cdc25 protein contains an intrinsic phosphatase activity.
    Cell. 1991 Oct 4;67(1):189-96 PMID: 1655274
  31. A negative regulator of mitosis in Aspergillus is a putative membrane-spanning protein.
    J Biol Chem. 1990 Sep 25;265(27):16132-7 PMID: 1697851
  32. Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase.
    Science. 1990 Dec 14;250(4987):1573-6 PMID: 1703321
  33. mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
    Cell. 1991 Mar 22;64(6):1111-22 PMID: 1706223
  34. Cyclin B targets p34cdc2 for tyrosine phosphorylation.
    EMBO J. 1991 Jun;10(6):1545-54 PMID: 1709096
  35. Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase.
    Nature. 1991 Feb 28;349(6312):808-11 PMID: 1825699
  36. The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
    Cell. 1991 Mar 8;64(5):903-14 PMID: 1825803
  37. Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
    Nature. 1991 May 16;351(6323):242-5 PMID: 1828290
  38. Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.
    Cell. 1991 Dec 20;67(6):1181-94 PMID: 1836978
  39. Cyclin is degraded by the ubiquitin pathway.
    Nature. 1991 Jan 10;349(6305):132-8 PMID: 1846030
  40. Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.
    EMBO J. 1991 Feb;10(2):305-16 PMID: 1846803
  41. A Tyr/Ser protein phosphatase encoded by vaccinia virus.
    Nature. 1991 Mar 28;350(6316):359-62 PMID: 1848923
  42. Mitotic control.
    Curr Opin Cell Biol. 1991 Apr;3(2):269-75 PMID: 1883620
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-06-00
Pages
2139-49
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556681
Subset
IM
Grants
NIGMS NIH HHS · GM34711 · United States
NIGMS NIH HHS · GM42564 · United States
Databases
GENBANK
X64555, X64556, X64601, X64602, X64629, X64700, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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