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PMID: 15351703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon.

Biochemical and biophysical research communications ·Vol. 323 ·No. 1 ·2004-10-08 ·Pages 72-8

Akashi K, Nishimura N, Ishida Y, Yokota A

Abstract

Wild watermelon (Citrullus lanatus sp.) has the ability to tolerate severe drought/high light stress conditions despite carrying out normal C3-type photosynthesis. Here, mRNA differential display was employed to isolate drought-responsive genes in the leaves of wild watermelon. One of the isolated genes, CLMT2, shared significant homology with type-2 metallothionein (MT) sequences from other plants. The second-order rate constant for the reaction between a recombinant CLMT2 protein and hydroxyl radicals was estimated to be 1.2 x 10(11) M(-1) s(-1), demonstrating that CLMT2 had an extraordinary high activity for detoxifying hydroxyl radicals. Moreover, hydroxyl radical-catalyzed degradation of watermelon genomic DNA was effectively suppressed by CLMT2 in vitro. This is the first demonstration of a plant MT with antioxidant properties. The results suggest that CLMT2 induction contributes to the survival of wild watermelon under severe drought/high light stress conditions.

MeSH Terms

Amino Acid Sequence Arabidopsis Proteins/chemistry,metabolism Catalysis Citrullus Cloning, Molecular DNA/chemistry DNA, Complementary/metabolism Disasters Dose-Response Relationship, Drug Gene Expression Profiling Hydroxyl Radical/chemistry Metallothionein/chemistry,metabolism Molecular Sequence Data Oxidative Stress Phylogeny Plant Proteins/chemistry RNA/chemistry RNA, Messenger/metabolism Recombinant Proteins/chemistry Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Factors

Chemicals

Arabidopsis Proteins DNA, Complementary MT2A protein, Arabidopsis Plant Proteins RNA, Messenger Recombinant Proteins Hydroxyl Radical RNA DNA Metallothionein

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Akashi Kinya

Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan. [email protected]

Nishimura Noriyuki

Ishida Yoshinori

Yokota Akiho

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/j.bbrc.2004.08.056

[email protected]

Published

2004-10-08

Pages

72-8

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

AB182918, AB182919, AB182920, AB182921, AB182922, AB182923, AB182924, AB182925, AB182926, AB182927, AB182928, AB182929, AB182930, AB182931, AB182932, AB182933, AB182934, AB182935, AB182936, AB182937, AB182938

External Links

PubMed source DOI: 10.1016/j.bbrc.2004.08.056 View journal details

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Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon.

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