Home LiteratureArticle Details
PMID: 15356392 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis.

Plant molecular biology ·Vol. 54 ·No. 5 ·2004-03-00 ·Pages 743-53

Puhakainen T, Hess MW, Mäkelä P, Svensson J, Heino P, Palva ET

Abstract

To elucidate the contribution of dehydrins (DHNs) to freezing stress tolerance in Arabidopsis, transgenic plants overexpressing multiple DHN genes were generated. Chimeric double constructs for expression of RAB18 and COR47 (pTP9) or LTI29 and LTI30 (pTP10) were made by fusing the coding sequences of the respective DHN genes to the cauliflower mosaic virus 35S promoter. Overexpression of the chimeric genes in Arabidopsis resulted in accumulation of the corresponding dehydrins to levels similar or higher than in cold-acclimated wild-type plants. Transgenic plants exhibited lower LT50 values and improved survival when exposed to freezing stress compared to the control plants. Post-embedding immuno electron microscopy of high-pressure frozen, freeze-substituted samples revealed partial intracellular translocation from cytosol to the vicinity of the membranes of the acidic dehydrin LTI29 during cold acclimation in transgenic plants. This study provides evidence that dehydrins contribute to freezing stress tolerance in plants and suggests that this could be partly due to their protective effect on membranes.

MeSH Terms
Adaptation, Physiological/genetics Arabidopsis/genetics,growth & development,ultrastructure Freezing Gene Expression Regulation, Plant Microscopy, Immunoelectron Plant Leaves/genetics,growth & development,ultrastructure Plant Proteins/genetics Plants, Genetically Modified
Chemicals
Plant Proteins dehydrin proteins, plant
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Puhakainen Tuula
Genetics, Department of Biological and Environmental Sciences, Viikki Biocenter, P.O. Box 56, FIN-00014, University of Helsinki, Finland.
Hess Michael W
Mäkelä Pirjo
Svensson Jan
Heino Pekka
Palva E Tapio
References (40)
40 references, click to expand
  1. The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh.
    Plant Mol Biol. 1992 Dec;20(5):951-62 PMID: 1463831
  2. Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation.
    J Biol Chem. 2003 Oct 17;278(42):40882-9 PMID: 12917402
  3. Expression of plant group 2 and group 3 lea genes in Saccharomyces cerevisiae revealed functional divergence among LEA proteins.
    J Biochem. 2000 Apr;127(4):611-6 PMID: 10739953
  4. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor.
    Nat Biotechnol. 1999 Mar;17(3):287-91 PMID: 10096298
  5. Promoter and expression studies on an Arabidopsis thaliana dehydrin gene.
    FEBS Lett. 1996 Mar 4;381(3):252-6 PMID: 8601466
  6. Immunolocalization of freezing-tolerance-associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues.
    Plant J. 1995 Oct;8(4):583-93 PMID: 7496403
  7. Differential expression of a gene encoding an acidic dehydrin in chilling sensitive and freezing tolerant gramineae species.
    FEBS Lett. 1994 May 9;344(1):20-4 PMID: 7910142
  8. Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco.
    Plant Mol Biol. 1992 Nov;20(3):555-8 PMID: 1421157
  9. Structure and organization of two closely related low-temperature-induced dhn/lea/rab-like genes in Arabidopsis thaliana L. Heynh.
    Plant Mol Biol. 1995 Oct;29(2):391-5 PMID: 7579189
  10. The wheat LEA protein Em functions as an osmoprotective molecule in Saccharomyces cerevisiae.
    Plant Mol Biol. 1999 Jan;39(1):117-28 PMID: 10080714
  11. Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize.
    Plant Mol Biol. 1990 Mar;14(3):423-32 PMID: 2151715
  12. Plant productivity and environment.
    Science. 1982 Oct 29;218(4571):443-8 PMID: 17808529
  13. Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana.
    Plant Mol Biol. 2001 Feb;45(3):263-79 PMID: 11292073
  14. Role of Abscisic Acid in Drought-Induced Freezing Tolerance, Cold Acclimation, and Accumulation of LT178 and RAB18 Proteins in Arabidopsis thaliana.
    Plant Physiol. 1995 Jan;107(1):141-148 PMID: 12228349
  15. Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice.
    Plant Physiol. 1996 Jan;110(1):249-257 PMID: 12226181
  16. Characterization of two cDNAs (ERD10 and ERD14) corresponding to genes that respond rapidly to dehydration stress in Arabidopsis thaliana.
    Plant Cell Physiol. 1994 Mar;35(2):225-31 PMID: 8069491
  17. PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms.
    Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599 PMID: 15012220
  18. Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins. Molecular characterization and functional analyses.
    Plant Physiol. 2002 Jul;129(3):1368-81 PMID: 12114590
  19. Of plants and other pets: practical aspects of freeze-substitution and resin embedding.
    J Microsc. 2003 Oct;212(Pt 1):44-52 PMID: 14516361
  20. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
    Plant J. 2002 Aug;31(3):279-92 PMID: 12164808
  21. Dehydrins in cold-acclimated apices of birch (Betula pubescens ehrh. ): production, localization and potential role in rescuing enzyme function during dehydration
    Planta. 1999 Oct;209(4):377-88 PMID: 10550618
  22. Characterization and differential expression of dhn/lea/rab-like genes during cold acclimation and drought stress in Arabidopsis thaliana.
    Plant Mol Biol. 1994 Oct;26(1):131-44 PMID: 7948863
  23. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway.
    Plant Cell. 2002 Aug;14(8):1675-90 PMID: 12172015
  24. The calcium-binding activity of a vacuole-associated, dehydrin-like protein is regulated by phosphorylation.
    Plant Physiol. 2002 Oct;130(2):675-87 PMID: 12376635
  25. Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope.
    EMBO J. 1992 Jun;11(6):2345-55 PMID: 1376250
  26. Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene.
    Plant Sci. 2000 Jun 12;155(1):1-9 PMID: 10773334
  27. Low temperature acclimation and treatment with exogenous abscisic acid induce common polypeptides in Arabidopsis thaliana (L.) Heynh.
    Theor Appl Genet. 1989 May;77(5):729-34 PMID: 24232808
  28. Characterization of a gene for spinach CAP160 and expression of two spinach cold-acclimation proteins in tobacco.
    Plant Physiol. 1998 Apr;116(4):1367-77 PMID: 9536054
  29. Eskimo1 mutants of Arabidopsis are constitutively freezing-tolerant.
    Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7799-804 PMID: 9636231
  30. A lea-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae.
    Gene. 1996 May 8;170(2):243-8 PMID: 8666253
  31. The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity.
    Plant Physiol. 2003 Jan;131(1):309-16 PMID: 12529538
  32. Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions).
    Plant Physiol. 1995 Sep;109(1):15-30 PMID: 12228580
  33. Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco.
    Planta. 2003 Jun;217(2):290-8 PMID: 12783337
  34. Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat
    Plant Cell. 1998 Apr;10(4):623-38 PMID: 9548987
  35. Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation.
    Plant Physiol. 2000 Dec;124(4):1854-65 PMID: 11115899
  36. Mass spectrometric approach for identifying putative plasma membrane proteins of Arabidopsis leaves associated with cold acclimation.
    Plant J. 2003 Oct;36(2):141-54 PMID: 14535880
  37. THE MOLECULAR BASIS OF DEHYDRATION TOLERANCE IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:377-403 PMID: 15012294
  38. Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance.
    Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13404-9 PMID: 11038526
  39. Purification of recombinant Arabidopsis thaliana dehydrins by metal ion affinity chromatography.
    Protein Expr Purif. 2000 Nov;20(2):169-78 PMID: 11049741
  40. Structure and sequence of a dehydrin-like gene in Arabidopsis thaliana.
    Plant Mol Biol. 1992 Jun;19(3):531-2 PMID: 1623199
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/B:PLAN.0000040903.66496.a4
Published
2004-03-00
Pages
743-53
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/B:PLAN.0000040903.66496.a4 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2