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PMID: 15356394 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities.

Plant molecular biology ·Vol. 54 ·No. 5 ·2004-03-00 ·Pages 767-81

Gilmour SJ, Fowler SG, Thomashow MF

Abstract

When Arabidopsis is exposed to low temperature a small gene family encoding transcription factors known as CBF1, CBF2, and CBF3 (also referred to as DREB1b, DREB1c, and DREB1a, respectively) is rapidly induced followed by expression of CBF-targeted genes, the CBF regulon, which act to bring about an increase in freezing tolerance. The CBF1, 2 and 3 proteins, though highly similar in amino acid sequence, are not identical, raising the question of whether the proteins have the same functions. Here we explored this issue by comparing the effects that overexpression of each CBF gene had on Arabidopsis growth and development, proline and sugar composition, freezing tolerance and gene expression. Taken together, the results support the conclusion that the CBF1, 2 and 3 transcriptional activators have redundant functional activities.

MeSH Terms
Adaptation, Physiological/genetics Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Blotting, Northern DNA-Binding Proteins/genetics,metabolism Electrophoresis, Polyacrylamide Gel Flowers/genetics,growth & development,metabolism Freezing Fructose/metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Glucose/metabolism Glycoside Hydrolases/metabolism Microscopy, Confocal Phenotype Plants, Genetically Modified Proline/metabolism Sucrose/metabolism Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins CBF1 protein, Arabidopsis CBF2 protein, Arabidopsis DNA-Binding Proteins DREB1A protein, Arabidopsis Trans-Activators Transcription Factors Fructose Sucrose Proline Glycoside Hydrolases raffinase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilmour Sarah J
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Fowler Sarah G
Thomashow Michael F
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/B:PLAN.0000040902.06881.d4
Published
2004-03-00
Pages
767-81
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/B:PLAN.0000040902.06881.d4 View journal details
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