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PMID: 15371544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Disruption of yeast forkhead-associated cell cycle transcription by oxidative stress.

Molecular biology of the cell ·Vol. 15 ·No. 12 ·2004-12-00 ·Pages 5659-69

Shapira M, Segal E, Botstein D

Abstract

The effects of oxidative stress on yeast cell cycle depend on the stress-exerting agent. We studied the effects of two oxidative stress agents, hydrogen peroxide (HP) and the superoxide-generating agent menadione (MD). We found that two small coexpressed groups of genes regulated by the Mcm1-Fkh2-Ndd1 transcription regulatory complex are sufficient to account for the difference in the effects of HP and MD on the progress of the cell cycle, namely, G1 arrest with MD and an S phase delay followed by a G2/M arrest with HP. Support for this hypothesis is provided by fkh1fkh2 double mutants, which are affected by MD as we find HP affects wild-type cells. The apparent involvement of a forkhead protein in HP-induced cell cycle arrest, similar to that reported for Caenorhabditis elegans and human, describes a potentially novel stress response pathway in yeast.

MeSH Terms
Cell Cycle/drug effects,genetics Cell Cycle Proteins/genetics,metabolism Forkhead Transcription Factors Gene Expression Profiling Gene Expression Regulation, Fungal/drug effects Hydrogen Peroxide/pharmacology Oxidative Stress/drug effects Saccharomyces cerevisiae/cytology,drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic/drug effects,genetics Vitamin K 3/pharmacology
Chemicals
Cell Cycle Proteins Fkh1 protein, S cerevisiae Fkh2 protein, S cerevisiae Forkhead Transcription Factors Saccharomyces cerevisiae Proteins Transcription Factors Vitamin K 3 Hydrogen Peroxide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shapira Michael
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA. [email protected]
Segal Eran
Botstein David
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-04-0340
Email
[email protected]
Published
2004-12-00
Epub
2004-00-15
Pages
5659-69
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC532044
Subset
IM
Grants
NIGMS NIH HHS · R01 GM046406 · United States
NIGMS NIH HHS · R37 GM046406 · United States
NIGMS NIH HHS · GM-46406 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1091/mbc.e04-04-0340 PMC Full Text View journal details
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