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PMID: 1537791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multicopy suppression: an approach to understanding intracellular functioning of the protein export system.

Journal of bacteriology ·Vol. 174 ·No. 5 ·1992-03-00 ·Pages 1454-61

Ueguchi C, Ito K

Abstract

Escherichia coli genes were cloned onto a multicopy plasmid and selected by the ability to restore growth and protein export defects caused by a temperature-sensitive secY or secA mutation. When secA51 was used as the primary mutation, only clones carrying groE, which specifies the chaperonin class of heat shock protein, were obtained. Selection using secY24 yielded three major classes of genes. The first class encodes another heat shock protein, HtpG; the most frequently obtained second class encodes a neutral histonelike protein, H-NS; and the third class, msyB, encodes a 124-residue protein of which 38 residues are acidic amino acids. Possible mechanisms of suppression as well as the significance and limitations of the multicopy suppression approach are discussed.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/genetics,metabolism Base Sequence Biological Transport, Active/genetics Chaperonins DNA-Binding Proteins/metabolism Escherichia coli/genetics Escherichia coli Proteins Genetic Complementation Test HSP90 Heat-Shock Proteins Heat-Shock Proteins/genetics,metabolism Molecular Sequence Data Multigene Family Plasmids/genetics Protein Processing, Post-Translational Suppression, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins GroE protein, E coli H-NS protein, bacteria HSP90 Heat-Shock Proteins Heat-Shock Proteins HtpG protein, E coli HtpG protein, bacteria msyB protein, E coli Chaperonins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ueguchi C
Institute for Virus Research, Kyoto University, Japan.
Ito K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.5.1454-1461.1992
Published
1992-03-00
Pages
1454-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206540
Subset
IM
Databases
GENBANK
M84772, M92999, M93000, M93001, M93002, M93003, M98830, X56583, X59939, X59940
External Links
PubMed source DOI: 10.1128/jb.174.5.1454-1461.1992 PMC Full Text View journal details
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