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PMID: 15395569 Published · ppublish English Journal Article

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

The Journal of experimental medicine ·Vol. 91 ·No. 1 ·1950-01-01 ·Pages 1-13

COONS AH, KAPLAN MH

Abstract

Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

Keywords
ANTIGENS AND ANTIBODIES
MeSH Terms
Antibodies Antigens Fluorescent Antibody Technique Humans Immunoglobulins
Chemicals
Antibodies Antigens Immunoglobulins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
COONS A H
KAPLAN M H
References (6)
6 references, click to expand
  1. The Zone of Localization of Anti-Mouse-Kidney Serum as Determined by Radioautographs.
    Science. 1949 Jan 21;109(2821):65-6 PMID: 17777137
  2. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse.
    J Exp Med. 1950 Jan 1;91(1):15-30, 4 pl PMID: 15395570
  3. Localization of antigen in tissue cells; antigens of rickettsiae and mumps virus.
    J Exp Med. 1950 Jan 1;91(1):31-8, pl PMID: 15395571
  4. Phenyl isocyanate protein compounds and their immunological properties.
    Biochem J. 1933;27(3):740-53 PMID: 16745152
  5. THE EFFECT OF COMBINATION WITH DIAZO COMPOUNDS ON THE IMMUNOLOGICAL REACTIVITY OF ANTIBODIES.
    J Exp Med. 1936 Apr 30;63(5):617-43 PMID: 19870493
  6. The Permeability of the Renal Glomeruli of Several Mammalian Species to Labelled Proteins.
    Am J Pathol. 1947 Mar;23(2):255-67 PMID: 19970929
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.91.1.1
Published
1950-01-01
Pages
1-13
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2135948
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.91.1.1 PMC Full Text View journal details
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