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PMID: 15466567 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of the groEL and groES loci in Bifidobacterium breve UCC 2003: genetic, transcriptional, and phylogenetic analyses.

Applied and environmental microbiology ·Vol. 70 ·No. 10 ·2004-10-00 ·Pages 6197-209

Ventura M, Canchaya C, Zink R, Fitzgerald GF, van Sinderen D

Abstract

The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes, including the GroEL and GroES proteins. The groES and groEL genes are highly conserved among eubacteria and are typically arranged as an operon. Genome analysis of Bifidobacterium breve UCC 2003 revealed that the groES and groEL genes are located in different chromosomal regions. The heat inducibility of the groEL and groES genes of B. breve UCC 2003 was verified by slot blot analysis. Northern blot analyses showed that the cspA gene is cotranscribed with the groEL gene, while the groES gene is transcribed as a monocistronic unit. The transcription initiation sites of these two mRNAs were determined by primer extension. Sequence and transcriptional analyses of the region flanking the groEL and groES genes of various bifidobacteria revealed similar groEL-cspA and groES gene units, suggesting a novel genetic organization of these chaperones. Phylogenetic analysis of the available bifidobacterial groES and groEL genes suggested that these genes evolved differently. Discrepancies in the phylogenetic positioning of groES-based trees make this gene an unreliable molecular marker. On the other hand, the bifidobacterial groEL gene sequences can be used as an alternative to current methods for tracing Bifidobacterium species, particularly because they allow a high level of discrimination between closely related species of this genus.

MeSH Terms
Base Sequence Bifidobacterium/classification,genetics Chaperonin 10/genetics Chaperonin 60/genetics Cloning, Molecular DNA, Bacterial/genetics Gene Dosage Genes, Bacterial Heat-Shock Response/genetics Molecular Sequence Data Nucleic Acid Conformation Phylogeny RNA, Bacterial/chemistry,genetics RNA, Ribosomal, 16S/genetics Species Specificity Transcription, Genetic
Chemicals
Chaperonin 10 Chaperonin 60 DNA, Bacterial RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ventura Marco
Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland. [email protected].
Canchaya Carlos
Zink Ralf
Fitzgerald Gerald F
van Sinderen Douwe
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.70.10.6197-6209.2004
Email
[email protected]
Published
2004-10-00
Pages
6197-209
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC522111
Subset
IM
Databases
GENBANK
AY585261, AY585262, AY586538, AY586539
External Links
PubMed source DOI: 10.1128/AEM.70.10.6197-6209.2004 PMC Full Text View journal details
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