Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats.

Infection and immunity ·Vol. 60 ·No. 4 ·1992-04-00 ·Pages 1268-72

Iida M, Watanabe K, Tsurufuji M, Takaishi K, Iizuka Y, Tsurufuji S

Abstract

Rat cytokine-induced neutrophil chemoattractant (CINC), which is a counterpart of human gro and belongs to the interleukin-8 family, has been quantified by a new sandwich enzyme-linked immunosorbent assay. Administration of lipopolysaccharide (LPS) into an air pouch performed by subcutaneous injection of air caused inflammation and severe neutrophil infiltration. After the LPS injection, changes in the concentration of CINC/gro, chemotactic activity, and the number of neutrophils in the air pouch exudate were determined. The chemotactic activity of neutrophils was augmented before practical neutrophil infiltration. More than half of the chemotactic activity was neutralized by the antisera. The time kinetics of the level of CINC/gro coincided with the changes in chemotactic activity. The maximal level of rat CINC/gro was 85 ng/ml, which is sufficient to cause neutrophil migration in vitro and in vivo as described previously. These data suggest that rat CINC/gro is a functional chemoattractant for neutrophils in LPS-induced inflammation in rats.

MeSH Terms

Animals Chemokine CXCL1 Chemokines, CXC Chemotactic Factors/immunology Chemotaxis, Leukocyte Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay Growth Substances/immunology Immunodiffusion Inflammation/chemically induced,immunology,pathology Injections, Subcutaneous Intercellular Signaling Peptides and Proteins Lipopolysaccharides Neutrophils/physiology Rats Rats, Inbred Strains Time Factors

Chemicals

CXCL1 protein, human Chemokine CXCL1 Chemokines, CXC Chemotactic Factors Cxcl1 protein, rat Growth Substances Intercellular Signaling Peptides and Proteins Lipopolysaccharides

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Iida M

Institute of Cytosignal Research Inc., Tokyo, Japan.

Watanabe K

Tsurufuji M

Takaishi K

Iizuka Y

Tsurufuji S

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.60.4.1268-1272.1992

Published

1992-04-00

Pages

1268-72

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC256992

Subset

External Links

PubMed source DOI: 10.1128/iai.60.4.1268-1272.1992 PMC Full Text View journal details

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Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats.

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