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PMID: 15496928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1.

Nature ·Vol. 431 ·No. 7011 ·2004-10-21 ·Pages 1011-7

Ira G, Pellicioli A, Balijja A, Wang X, Fiorani S, Carotenuto W, Liberi G, Bressan D, Wan L, Hollingsworth NM, Haber JE, Foiani M

Abstract

A single double-strand break (DSB) induced by HO endonuclease triggers both repair by homologous recombination and activation of the Mec1-dependent DNA damage checkpoint in budding yeast. Here we report that DNA damage checkpoint activation by a DSB requires the cyclin-dependent kinase CDK1 (Cdc28) in budding yeast. CDK1 is also required for DSB-induced homologous recombination at any cell cycle stage. Inhibition of homologous recombination by using an analogue-sensitive CDK1 protein results in a compensatory increase in non-homologous end joining. CDK1 is required for efficient 5' to 3' resection of DSB ends and for the recruitment of both the single-stranded DNA-binding complex, RPA, and the Rad51 recombination protein. In contrast, Mre11 protein, part of the MRX complex, accumulates at unresected DSB ends. CDK1 is not required when the DNA damage checkpoint is initiated by lesions that are processed by nucleotide excision repair. Maintenance of the DSB-induced checkpoint requires continuing CDK1 activity that ensures continuing end resection. CDK1 is also important for a later step in homologous recombination, after strand invasion and before the initiation of new DNA synthesis.

MeSH Terms
CDC2 Protein Kinase/metabolism Cell Cycle/drug effects Cell Cycle Proteins/metabolism Checkpoint Kinase 2 DNA Damage/drug effects DNA Repair/genetics DNA, Fungal/genetics,metabolism Genes, Fungal/genetics Mating Factor Nocodazole/pharmacology Peptides/pharmacology Protein Serine-Threonine Kinases/metabolism Recombination, Genetic/genetics Saccharomyces cerevisiae/drug effects,enzymology,genetics Saccharomyces cerevisiae Proteins/metabolism Sequence Homology, Nucleic Acid
Chemicals
Cell Cycle Proteins DNA, Fungal Peptides Saccharomyces cerevisiae Proteins Mating Factor Checkpoint Kinase 2 Protein Serine-Threonine Kinases CDC2 Protein Kinase RAD53 protein, S cerevisiae Nocodazole
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Ira Grzegorz
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454-9110, USA.
Pellicioli Achille
Balijja Alitukiriza
Wang Xuan
Fiorani Simona
Carotenuto Walter
Liberi Giordano
Bressan Debra
Wan Lihong
Hollingsworth Nancy M
Haber James E
Foiani Marco
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
Published
2004-10-21
Pages
1011-7
Language
English
Region
England
NLM ID
0410462
PMCID
PMC4493751
Subset
IM
Grants
NIAID NIH HHS · AI44009 · United States
NIGMS NIH HHS · R01 GM050717 · United States
Telethon · GGP030412 · Italy
NIAID NIH HHS · R01 AI044009 · United States
NIGMS NIH HHS · R01 GM080600 · United States
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