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PMID: 15504916 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.

The Journal of cell biology ·Vol. 167 ·No. 2 ·2004-10-25 ·Pages 377-88

Carman CV, Springer TA

Abstract

The basic route and mechanisms for leukocyte migration across the endothelium remain poorly defined. We provide definitive evidence for transcellular (i.e., through individual endothelial cells) diapedesis in vitro and demonstrate that virtually all, both para- and transcellular, diapedesis occurs in the context of a novel "cuplike" transmigratory structure. This endothelial structure was comprised of highly intercellular adhesion molecule-1- and vascular cell adhesion molecule-1-enriched vertical microvilli-like projections that surrounded transmigrating leukocytes and drove redistribution of their integrins into linear tracks oriented parallel to the direction of diapedesis. Disruption of projections was highly correlated with inhibition of transmigration. These findings suggest a novel mechanism, the "transmigratory cup", by which the endothelium provides directional guidance to leukocytes for extravasation.

MeSH Terms
Antigens, CD Cadherins/metabolism Caveolin 1 Caveolins/metabolism Cell Adhesion Cell Movement Cells, Cultured Endothelium, Vascular/cytology,metabolism Humans Image Processing, Computer-Assisted Intercellular Adhesion Molecule-1/metabolism Leukocytes/cytology,metabolism Leukocytes, Mononuclear/metabolism Microscopy, Electron, Transmission Microscopy, Fluorescence Monocytes/metabolism Neutrophils/metabolism Time Factors Vascular Cell Adhesion Molecule-1/metabolism
Chemicals
Antigens, CD Cadherins Caveolin 1 Caveolins Vascular Cell Adhesion Molecule-1 cadherin 5 Intercellular Adhesion Molecule-1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carman Christopher V
The CBR Institute for Biomedical Research, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Springer Timothy A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200404129
Published
2004-10-25
Pages
377-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172560
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200404129 PMC Full Text View journal details
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