The effects of thapsigargin on [Ca2+]i in isolated rat mesenteric artery vascular smooth muscle cells.
The effects of thapsigargin were studied on single cells isolated from side branches of the rat mesenteric artery. Thapsigargin (150 nM) produced a transient increase of [Ca2+]i. This transient rise of [Ca2+]i was unaffected by removing external Ca2+ ions. This suggests that thapsigargin is releasing Ca2+ ions from an intracellular store. In the absence of thapsigargin both noradrenaline and caffeine also produced a transient increase of [Ca2+]i. These increases were abolished by prior exposure to thapsigargin. Correspondingly, the effects of thapsigargin were abolished by prior exposure to caffeine. These results show that thapsigargin releases Ca2+ from the noradrenaline and caffeine-sensitive stores.
10 references, click to expand
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Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane.J Biol Chem. 1989 Jul 25;264(21):12266-71 PMID: 2663854
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Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.Proc Natl Acad Sci U S A. 1990 Apr;87(7):2466-70 PMID: 2138778
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[Ca2+]i and contraction of ear artery in response to rapid stimulation by NE: measurements with quin2 and fura-2.Am J Physiol. 1989 Aug;257(2 Pt 2):H649-57 PMID: 2504060
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Effects of thapsigargin in isolated rat thoracic aorta.Pharmacol Toxicol. 1988 Jan;62(1):7-11 PMID: 3128782
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The inflammatory and tumor-promoting sesquiterpene lactone, thapsigargin, activates platelets by selective mobilization of calcium as shown by protein phosphorylations.Biochim Biophys Acta. 1987 Jan 19;927(1):65-73 PMID: 3098302
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The role of [Ca2+]i and [Ca2+] sensitization in the caffeine contracture of rat myocytes: measurement of [Ca2+]i and [caffeine]i.J Physiol. 1990 Jun;425:55-70 PMID: 2213589
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Changes in cytosolic calcium level in vascular smooth muscle strip measured simultaneously with contraction using fluorescent calcium indicator fura 2.J Pharmacol Exp Ther. 1988 Jul;246(1):294-300 PMID: 2455792
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