Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community.

International journal of systematic and evolutionary microbiology ·Vol. 54 ·No. Pt 6 ·2004-11-00 ·Pages 2043-2047

Kato S, Haruta S, Cui ZJ, Ishii M, Yokota A, Igarashi Y

Abstract

A novel anaerobic, thermophilic and cellulolytic bacterium (strain CSK1(T)) was isolated from a cellulose-degrading bacterial community. On the basis of 16S rRNA gene sequence similarity, strain CSK1(T) was mapped to cluster III of the genus Clostridium. Strain CSK1(T) is closely related to Clostridium thermocellum (96.2 %) and Clostridium aldrichii (95.1 %). Strain CSK1(T) is a non-motile, spore-forming, straight or slightly curved rod. The optimum temperature and initial pH for its growth and cellulose degradation are 50-55 degrees C and pH 7.5. Strain CSK1(T) grew under a gas phase containing up to 4 % O(2). Phylogenetic and phenotypic analyses support the differentiation of strain CSK1(T) from its closest relatives. Strain CSK1(T) therefore represents a novel species, for which the name Clostridium straminisolvens sp. nov. is proposed, with CSK1(T) (=DSM 16021(T)=IAM 15070(T)) as the type strain.

MeSH Terms

Anaerobiosis Cellulose/metabolism Clostridium/classification,cytology,isolation & purification,physiology DNA, Bacterial/chemistry,isolation & purification DNA, Ribosomal/chemistry,isolation & purification Genes, rRNA Hydrogen-Ion Concentration Molecular Sequence Data Movement Oxygen/toxicity Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Spores, Bacterial/cytology Temperature

Chemicals

DNA, Bacterial DNA, Ribosomal RNA, Bacterial RNA, Ribosomal, 16S Cellulose Oxygen

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kato Souichiro