Abstract
The purpose of this study was to identify the biochemical and genetic defect in L-2-hydroxyglutaric aciduria, a neurometabolic disorder characterized by the presence of elevated concentrations of L-2-hydroxyglutaric acid in urine, plasma, and cerebrospinal fluid. Evidence is provided for the existence in rat tissues of a FAD-dependent enzyme catalyzing specifically the oxidation of L-2-hydroxyglutarate to alpha-ketoglutarate. This enzyme is mainly expressed in liver and kidney but also at lower levels in heart, brain, and other tissues. Subcellular fractionation indicates that the liver enzyme is present in mitochondria, where it is bound to membranes. Based on this information, a database search led to the identification of a gene encoding a human hypothetical protein homologous to bacterial FAD-dependent malate dehydrogenases and targeted to mitochondria. The gene encoding this protein, present on chromosome 14q22.1, was found to be in a region homozygous in patients with L-2-hydroxyglutaric aciduria from two consanguineous families. Three mutations that replaced a highly conserved residue (Lys-71-Glu and Glu-176-Asp) or removed exon 9 were identified in homozygous state in patients from three distinct families and were found to cosegregate with the disease. It is concluded that L-2-hydroxyglutarate is normally metabolized to alpha-ketoglutarate in mammalian tissues and that L-2-hydroxyglutaric aciduria is caused by mutations in the gene that most likely encodes L-2-hydroxyglutarate dehydrogenase. The pathological findings observed in this metabolic disorder must therefore be due to a toxic effect of L-2-hydroxyglutarate on the central nervous system.
MeSH Terms
Alcohol Oxidoreductases/chemistry,genetics,metabolism
Amino Acid Sequence
Female
Flavin-Adenine Dinucleotide/metabolism
Glutarates/urine
Humans
Male
Molecular Sequence Data
Mutation
Pedigree
Sequence Homology, Amino Acid
Subcellular Fractions/enzymology
Chemicals
Glutarates
Flavin-Adenine Dinucleotide
alpha-hydroxyglutarate
Alcohol Oxidoreductases
2-hydroxyglutarate dehydrogenase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rzem Rim
Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology, Université Catholique de Louvain, Avenue Hippocrate 75, B-1200 Brussels, Belgium.
Veiga-da-Cunha Maria
Noël Gaëtane
Goffette Sophie
Nassogne Marie-Cécile
Tabarki Brahim
Schöller Christina
Marquardt Thorsten
Vikkula Miikka
Van Schaftingen Emile
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