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PMID: 15548746 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The role of [Delta]1-pyrroline-5-carboxylate dehydrogenase in proline degradation.

The Plant cell ·Vol. 16 ·No. 12 ·2004-12-00 ·Pages 3413-25

Deuschle K, Funck D, Forlani G, Stransky H, Biehl A, Leister D, van der Graaff E, Kunze R, Frommer WB

Abstract

In response to stress, plants accumulate Pro, requiring degradation after release from adverse conditions. Delta1-Pyrroline-5-carboxylate dehydrogenase (P5CDH), the second enzyme for Pro degradation, is encoded by a single gene expressed ubiquitously. To study the physiological function of P5CDH, T-DNA insertion mutants in AtP5CDH were isolated and characterized. Although Pro degradation was undetectable in p5cdh mutants, neither increased Pro levels nor an altered growth phenotype were observed under normal conditions. Thus AtP5CDH is essential for Pro degradation but not required for vegetative plant growth. External Pro application caused programmed cell death, with callose deposition, reactive oxygen species production, and DNA laddering, involving a salicylic acid signal transduction pathway. p5cdh mutants were hypersensitive toward Pro and other molecules producing P5C, such as Arg and Orn. Pro levels were the same in the wild type and mutants, but P5C was detectable only in p5cdh mutants, indicating that P5C accumulation may be the cause for Pro hypersensitivity. Accordingly, overexpression of AtP5CDH resulted in decreased sensitivity to externally supplied Pro. Thus, Pro and P5C/Glu semialdehyde may serve as a link between stress responses and cell death.

MeSH Terms
1-Pyrroline-5-Carboxylate Dehydrogenase Aldehyde Oxidoreductases/metabolism Apoptosis/drug effects,physiology Arabidopsis/drug effects,enzymology,genetics Arabidopsis Proteins/genetics,metabolism Arginine/metabolism,pharmacology Gene Expression Regulation, Enzymologic/drug effects,genetics Gene Expression Regulation, Plant/drug effects,genetics Glutamate-5-Semialdehyde Dehydrogenase Glutamic Acid/metabolism Mutation/genetics Ornithine/metabolism,pharmacology Oxidoreductases Acting on CH-NH Group Donors/genetics,metabolism Proline/metabolism,pharmacology Reactive Oxygen Species/metabolism Salicylic Acid/metabolism Signal Transduction/drug effects,physiology
Chemicals
Arabidopsis Proteins Reactive Oxygen Species Glutamic Acid Arginine Proline Ornithine Aldehyde Oxidoreductases Glutamate-5-Semialdehyde Dehydrogenase 1-Pyrroline-5-Carboxylate Dehydrogenase Oxidoreductases Acting on CH-NH Group Donors Salicylic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Deuschle Karen
Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, D-72076 Tübingen, Germany.
Funck Dietmar
Forlani Giuseppe
Stransky Harald
Biehl Alexander
Leister Dario
van der Graaff Eric
Kunze Reinhard
Frommer Wolf B
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.023622
Published
2004-12-00
Epub
2004-00-17
Pages
3413-25
Language
English
Region
England
NLM ID
9208688
PMCID
PMC535882
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.104.023622 PMC Full Text View journal details
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