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PMID: 15557118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Condensin restructures chromosomes in preparation for meiotic divisions.

The Journal of cell biology ·Vol. 167 ·No. 4 ·2004-11-22 ·Pages 613-25

Chan RC, Severson AF, Meyer BJ

Abstract

The production of haploid gametes from diploid germ cells requires two rounds of meiotic chromosome segregation after one round of replication. Accurate meiotic chromosome segregation involves the remodeling of each pair of homologous chromosomes around the site of crossover into a highly condensed and ordered structure. We showed that condensin, the protein complex needed for mitotic chromosome compaction, restructures chromosomes during meiosis in Caenorhabditis elegans. In particular, condensin promotes both meiotic chromosome condensation after crossover recombination and the remodeling of sister chromatids. Condensin helps resolve cohesin-independent linkages between sister chromatids and alleviates recombination-independent linkages between homologues. The safeguarding of chromosome resolution by condensin permits chromosome segregation and is crucial for the formation of discrete, individualized bivalent chromosomes.

MeSH Terms
Adenosine Triphosphatases/genetics,physiology Animals Caenorhabditis elegans/genetics,metabolism Caenorhabditis elegans Proteins/genetics,metabolism Cell Cycle Proteins/genetics,metabolism Cell Nucleus/genetics,metabolism Chromosome Segregation/genetics,physiology Chromosome Structures/genetics DNA-Binding Proteins/genetics,physiology Gametogenesis/genetics Meiosis/genetics,physiology Meiotic Prophase I/genetics Multiprotein Complexes Mutation/genetics Recombination, Genetic/genetics
Chemicals
Caenorhabditis elegans Proteins Cell Cycle Proteins DNA-Binding Proteins HCP-6 protein, C elegans MIX-1 protein, C elegans Multiprotein Complexes condensin complexes Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chan Raymond C
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Severson Aaron F
Meyer Barbara J
References (39)
39 references, click to expand
  1. DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.
    Cell. 1994 Nov 4;79(3):459-74 PMID: 7954812
  2. In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin.
    J Cell Biol. 2002 Mar 4;156(5):805-15 PMID: 11864994
  3. Condensin architecture and interaction with DNA: regulatory non-SMC subunits bind to the head of SMC heterodimer.
    Curr Biol. 2002 Mar 19;12(6):508-13 PMID: 11909539
  4. C. elegans condensin promotes mitotic chromosome architecture, centromere organization, and sister chromatid segregation during mitosis and meiosis.
    Genes Dev. 2002 Mar 15;16(6):729-42 PMID: 11914278
  5. The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis.
    J Cell Biol. 2002 Apr 15;157(2):219-29 PMID: 11940606
  6. The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis.
    Curr Biol. 2002 May 14;12(10):798-812 PMID: 12015116
  7. Cellular roles of DNA topoisomerases: a molecular perspective.
    Nat Rev Mol Cell Biol. 2002 Jun;3(6):430-40 PMID: 12042765
  8. Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment.
    Genes Dev. 2002 Jun 15;16(12):1498-508 PMID: 12080088
  9. Segregating sister genomes: the molecular biology of chromosome separation.
    Science. 2002 Jul 26;297(5581):559-65 PMID: 12142526
  10. Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans.
    Genes Dev. 2002 Sep 15;16(18):2428-42 PMID: 12231631
  11. The making of the mitotic chromosome: modern insights into classical questions.
    Mol Cell. 2003 Mar;11(3):557-69 PMID: 12667441
  12. Rad50/SMC proteins and ABC transporters: unifying concepts from high-resolution structures.
    Curr Opin Struct Biol. 2003 Apr;13(2):249-55 PMID: 12727520
  13. Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis.
    Development. 2003 Jul;130(14):3283-95 PMID: 12783798
  14. Chromosome cohesion is regulated by a clock gene paralogue TIM-1.
    Nature. 2003 Jun 26;423(6943):1002-9 PMID: 12827206
  15. Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
    Cell. 2003 Oct 3;115(1):109-21 PMID: 14532007
  16. Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A.
    Curr Biol. 2003 Dec 2;13(23):2058-64 PMID: 14653995
  17. Meiotic condensin is required for proper chromosome compaction, SC assembly, and resolution of recombination-dependent chromosome linkages.
    J Cell Biol. 2003 Dec 8;163(5):937-47 PMID: 14662740
  18. MUSCLE: multiple sequence alignment with high accuracy and high throughput.
    Nucleic Acids Res. 2004;32(5):1792-7 PMID: 15034147
  19. Chromosome distribution: experiments on cell hybrids and in vitro.
    Philos Trans R Soc Lond B Biol Sci. 1977 Mar 21;277(955):267-76 PMID: 16290
  20. Genes that implement the hermaphrodite mode of dosage compensation in Caenorhabditis elegans.
    Genetics. 1989 Jan;121(1):57-76 PMID: 2917714
  21. Meiotic chromosome pairing in maize is associated with a novel chromatin organization.
    Cell. 1994 Mar 11;76(5):901-12 PMID: 8124724
  22. Coming undone: how to untangle a chromosome.
    Cell. 1994 Jul 1;77(7):955-7 PMID: 8020101
  23. Mitotic chromosome condensation.
    Annu Rev Cell Dev Biol. 1996;12:305-33 PMID: 8970729
  24. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  25. Chromosome segregation during meiosis: building an unambivalent bivalent.
    Curr Top Dev Biol. 1998;37:263-99 PMID: 9352189
  26. MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.
    Cell. 1998 Jan 23;92(2):265-77 PMID: 9458050
  27. Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis.
    Cell. 1998 Aug 7;94(3):387-98 PMID: 9708740
  28. On the control of oocyte meiotic maturation and ovulation in Caenorhabditis elegans.
    Dev Biol. 1999 Jan 1;205(1):111-28 PMID: 9882501
  29. A histone-H3-like protein in C. elegans.
    Nature. 1999 Oct 7;401(6753):547-8 PMID: 10524621
  30. Meiotic chromosomes: integrating structure and function.
    Annu Rev Genet. 1999;33:603-754 PMID: 10690419
  31. Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p.
    Genes Dev. 2000 Feb 15;14(4):493-503 PMID: 10691741
  32. T-Coffee: A novel method for fast and accurate multiple sequence alignment.
    J Mol Biol. 2000 Sep 8;302(1):205-17 PMID: 10964570
  33. The reduction of chromosome number in meiosis is determined by properties built into the chromosomes.
    J Cell Biol. 2000 Sep 18;150(6):1223-32 PMID: 10995430
  34. Dual roles of the 11S regulatory subcomplex in condensin functions.
    Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11972-7 PMID: 11027308
  35. Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.
    Genetics. 2001 Mar;157(3):1217-26 PMID: 11238406
  36. A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction.
    Genes Dev. 2001 Jun 1;15(11):1349-60 PMID: 11390355
  37. Functional analysis of kinetochore assembly in Caenorhabditis elegans.
    J Cell Biol. 2001 Jun 11;153(6):1209-26 PMID: 11402065
  38. HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes.
    J Cell Biol. 2001 Jun 11;153(6):1227-38 PMID: 11402066
  39. DPY-26, a link between dosage compensation and meiotic chromosome segregation in the nematode.
    Science. 1996 Dec 6;274(5293):1732-6 PMID: 8939869
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200408061
Published
2004-11-22
Pages
613-25
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172564
Subset
IM
Grants
NIGMS NIH HHS · R01 GM030702 · United States
NIGMS NIH HHS · R37 GM030702 · United States
NIGMS NIH HHS · GM30702 · United States
External Links
PubMed source DOI: 10.1083/jcb.200408061 PMC Full Text View journal details
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