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PMID: 15565466 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries.

Molecular genetics and genomics : MGG ·Vol. 272 ·No. 4 ·2004-11-00 ·Pages 480-7

Martínez Zamora MG, Castagnaro AP, Díaz Ricci JC

Abstract

Degenerate oligonucleotide primers, designed based on conserved regions of Nucleotide Binding Site (NBS) domains from previously cloned plant resistance genes, were used to isolate Resistance Gene Analogues (RGAs) from wild and cultivated strawberries. Seven distinct families of RGAs of the NBS-LRR type were identified from two related wild species, Fragaria vesca and F. chiloensis, and six different Fragaria x ananassa cultivars. With one exception (GAV-3), the deduced amino acid sequences of strawberry RGAs showed strong similarity to TIR (Toll Interleukin I Receptor)-type R genes from Arabidopsis, tobacco and flax, suggesting the existence of common ancestors. GAV-3 seemed to be more closely related to the non-TIR type. Further studies showed that the recombination level and the ratio of non-synonymous to synonymous substitutions within families were low. These data suggest that NBS-encoding sequences of RGAs in strawberry are subject to a gradual accumulation of mutations leading to purifying selection, rather than to a diversifying process. The present paper is the first report on RGAs in strawberry.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Cloning, Molecular Cluster Analysis Crosses, Genetic DNA Primers Fragaria/genetics,immunology Genes, Plant Genetic Variation Immunity, Innate/genetics Molecular Sequence Data Phylogeny Sequence Alignment Sequence Analysis, DNA
Chemicals
DNA Primers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martínez Zamora M G
Dpto. de Bioquímica de la Nutrición, Instituto de Química Biológica "Dr. Bernabé Bloj" (UNT), INSIBIO (CONICET-UNT), Chacabuco 461, 4000 Tucumán, Argentina.
Castagnaro A P
Díaz Ricci J C
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-004-1079-4
Published
2004-11-00
Epub
2004-00-23
Pages
480-7
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-004-1079-4 View journal details
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