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PMID: 15592428 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple activities contribute to Pc2 E3 function.

The EMBO journal ·Vol. 24 ·No. 1 ·2005-01-12 ·Pages 108-19

Kagey MH, Melhuish TA, Powers SE, Wotton D

Abstract

Pc2 is a polycomb protein, which has SUMO E3 activity for the corepressors CtBP and CtBP2. Here we demonstrate that, in vivo, Pc2 adapter function contributes to enhancement of CtBP sumoylation. Mutation of the CtBP binding site on Pc2 abolishes E3 activity toward CtBP. However, a carboxyl-terminal fragment of Pc2 that recruits both Ubc9 and CtBP lacks E3 activity. We identify a second domain, which, when coexpressed with the carboxyl-terminal adapter region, restores E3 function. In vitro, this domain has E3 activity in isolation, suggesting that it is a functional domain, and that adapter function is required to selectively corecruit E2 and substrate in vivo. These results demonstrate the presence of two domains in Pc2 that contribute to full in vivo E3 activity, and suggest that SUMO E3s are more than simple platforms to which E2 and substrate bind.

MeSH Terms
Alcohol Oxidoreductases Animals COS Cells Chlorocebus aethiops DNA-Binding Proteins/genetics,metabolism Phosphoproteins/genetics,metabolism Protein Structure, Tertiary Recombinant Fusion Proteins/genetics,metabolism Repressor Proteins/chemistry,genetics,metabolism Small Ubiquitin-Related Modifier Proteins/metabolism Ubiquitin-Conjugating Enzymes/genetics,metabolism Ubiquitin-Protein Ligases/genetics,metabolism
Chemicals
DNA-Binding Proteins Phosphoproteins Recombinant Fusion Proteins Repressor Proteins Small Ubiquitin-Related Modifier Proteins Alcohol Oxidoreductases C-terminal binding protein Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligases ubiquitin-conjugating enzyme UBC9
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kagey Michael H
Department of Biochemistry and Molecular Genetics, Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.
Melhuish Tiffany A
Powers Shannon E
Wotton David
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600506
Published
2005-01-12
Epub
2004-00-09
Pages
108-19
Language
English
Region
England
NLM ID
8208664
PMCID
PMC544918
Subset
IM
Grants
NICHD NIH HHS · R01 HD039926 · United States
NICHD NIH HHS · HD 39926 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7600506 PMC Full Text View journal details
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